Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182931_bp42 TAGCCTTCACCCAGAAGCATGCATGGCCTGAG(+CAGTGT)GTACCCATGGGTTATCTAACTTCTCAGAATC NGG 10 8 17 8 24 0 100 5 10
    240605182929_utvr GAGTGGATTCTGAGAAGTTAGATAACCCATGG(+CAGTGT)GTACCTCAGGCCATGCATGCTTCTGGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605182928_4wvb GCAATCTTCTTGCAATCCCTGACCTATTTGGG(+CAGTGT)GTACCTTAGAACCTCCTCTTGGCTAGAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605182926_avz2 AGCATCCTCTCTAGCCAAGAGGAGGTTCTAAG(+CAGTGT)GTACCCCAAATAGGTCAGGGATTGCAAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605182925_s834 GGCTGGCTGTGAGTCTACTTGGACTTTATCTG(+CAGTGT)GATCCTATAAGAACATTTACTCCTTGTTTAG NGG 10 8 17 8 24 0 100 5 10
    240605182924_52wl CCTGTCTAAACAAGGAGTAAATGTTCTTATAG(+CAGTGT)GATCCAGATAAAGTCCAAGTAGACTCACAGC NGG 10 8 17 8 24 0 100 5 10
    240605182922_ndsf CTGGCAGAGTGCTGGCATCCTCTAAATCCGTG(+CAGTGT)GTACCCAGGGCTACAGGCCCTGAGGCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605182918_r2j0 AGCCTTCCTTGCCTCAGGGCCTGTAGCCCTGG(+CAGTGT)GTACCACGGATTTAGAGGATGCCAGCACTCT NGG 10 8 17 8 24 0 100 5 10
    240605182916_5lho TGGTAAAGAGGTTTAAGATAGTTAATAATGGG(+CAGTGT)GTACCCCAGAAGCAAGAGTGTGTCAGGCAAA NGG 10 8 17 8 24 0 100 5 10
    240605182914_pe7m CTTATTTTGCCTGACACACTCTTGCTTCTGGG(+CAGTGT)GTACCCCATTATTAACTATCTTAAACCTCTT NGG 10 8 17 8 24 0 100 5 10