Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182842_4rgt CTGAGAAAGAGAAAGGTGTCTGAGTAATTCTG(+CAGTGT)GATCCAGTTTTTTCCCCTAGAAAGACTGAGA NGG 10 8 17 8 24 0 100 5 10
    240605182840_vrt4 GTAAGGAAGCAGAGCCCAGGAGCTCAAAGCTG(+CAGTGT)GTACCCAGAAGAGCCAGCAGGGCCAGGCTAA NGG 10 8 17 8 24 0 100 5 10
    240605182839_whri AGTCCTTAGCCTGGCCCTGCTGGCTCTTCTGG(+CAGTGT)GTACCAGCTTTGAGCTCCTGGGCTCTGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605182837_hzi9 GGTGACCTGCCATCTGTGACAAGCTTTCTCGG(+CAGTGT)GTACCTCTCCCCACCTCATTCCTTTAGGCTA NGG 10 8 17 8 24 0 100 5 10
    240605182836_es23 CTCCATAGCCTAAAGGAATGAGGTGGGGAGAG(+CAGTGT)GTACCCGAGAAAGCTTGTCACAGATGGCAGG NGG 10 8 17 8 24 0 100 5 10
    240605182835_t19v TGTAATTTTTGTATATAATGGGCCAAATTAAG(+CAGTGT)GATCCTGCTCTCTTTCTTTTGGAGGTAGTTT NGG 10 8 17 8 24 0 100 5 10
    240605182834_3igz ACCTCAAACTACCTCCAAAAGAAAGAGAGCAG(+CAGTGT)GATCCTTAATTTGGCCCATTATATACAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605182832_jp4e GCATGTATTCAGTTTTAGAATCACTTTTACTG(+CAGTGT)GATCCTGTAGTAATGTCTAGAAGTATCATAT NGG 10 8 17 8 24 0 100 5 10
    240605182831_8j0e AATAGATATGATACTTCTAGACATTACTACAG(+CAGTGT)GATCCAGTAAAAGTGATTCTAAAACTGAATA NGG 10 8 17 8 24 0 100 5 10
    240605182830_pyuj TTACTTGAAAATTGGACATAGTACTACCAGAG(+CAGTGT)GATCCCACAATACCTCTCCTGGGCATATATC NGG 10 8 17 8 24 0 100 5 10