Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182811_vl1a AGCCCAGAAACTGTCAGATCAACATAATATCG(+CAGTGT)GATCCTGTAAGTTTGGATTTTGTGCCTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605182810_o22u TGCTAAGCAGAATCAGGCTGTAGGAAGGCATG(+CAGTGT)GATCCTGGGGCAGGAACTGCACAGCCAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605182807_tlhh TCAGGGCCTTGGCTGTGCAGTTCCTGCCCCAG(+CAGTGT)GATCCATGCCTTCCTACAGCCTGATTCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605182805_pdul GACGTTGATGGATGACTTTTCCTTCATCCATG(+CAGTGT)GATCCCAAGAGTGGTGCAGTCCGCCAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605182803_542i GCGGTTCTCCTGGCGGACTGCACCACTCTTGG(+CAGTGT)GATCCATGGATGAAGGAAAAGTCATCCATCA NGG 10 8 17 8 24 0 100 5 10
    240605182801_vtwg GGTCCAAACAACACATCATCATGATGTCCCAG(+CAGTGT)GATCCACTATGTCCTCATTGCTTCCCAGAAT NGG 10 8 17 8 24 0 100 5 10
    240605182800_mwh9 GAAGGATTCTGGGAAGCAATGAGGACATAGTG(+CAGTGT)GATCCTGGGACATCATGATGATGTGTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240605182759_pvv3 GCTCAGGAAAGGGTGATAATGGATAGGATGCG(+CAGTGT)GATCCCTAAGTAGATGGGATAAATGTATTAC NGG 10 8 17 8 24 0 100 5 10
    240605182757_55he CGTGTGTAATACATTTATCCCATCTACTTAGG(+CAGTGT)GATCCGCATCCTATCCATTATCACCCTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605182755_wi61 ACCAGCCCAAGACATTTTTACTTTTCTCCTGG(+CAGTGT)GATCCAAAGAAATTCTGTGCTTCATGAGAAC NGG 10 8 17 8 24 0 100 5 10