Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182913_x0nt GACCATTGTAGCTCATAGTTAAATATGTTAAG(+CAGTGT)GATCCAGCATCTCAAAGATTAAAAAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240605182911_vnib TTTTTTTTATTTTTTTAATCTTTGAGATGCTG(+CAGTGT)GATCCTTAACATATTTAACTATGAGCTACAA NGG 10 8 17 8 24 0 100 5 10
    240605182910_gx0q TGCAGAGAAGAGGCTAATAATGAACTCAGAGG(+CAGTGT)GTACCTTGAAGACTGGTGTGCCAATGGATAC NGG 10 8 17 8 24 0 100 5 10
    240605182909_l4xi AACTAGTATCCATTGGCACACCAGTCTTCAAG(+CAGTGT)GTACCCTCTGAGTTCATTATTAGCCTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605182907_h1wm GTGACGCTCAGATCTGAGAAGAAACATGATGG(+CAGTGT)GATCCATATCAGGTTGTGAATATTTTTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605182906_ozlx TGTTCTCAAAAAAATATTCACAACCTGATATG(+CAGTGT)GATCCCATCATGTTTCTTCTCAGATCTGAGC NGG 10 8 17 8 24 0 100 5 10
    240605182904_3u44 TAAAGTACTATGACACTAGAACCTTTGCAGAG(+CAGTGT)GATCCAACTACAATGTAAGGTTGATCTTCCA NGG 10 8 17 8 24 0 100 5 10
    240605182903_zdpw GATACTGGAAGATCAACCTTACATTGTAGTTG(+CAGTGT)GATCCTCTGCAAAGGTTCTAGTGTCATAGTA NGG 10 8 17 8 24 0 100 5 10
    240605182901_ivqo AGAGCGGTCACTGGACTAGGATGCATCCCTTG(+CAGTGT)GATCCTGGGATAGGCTCTGATCGGACCATAA NGG 10 8 17 8 24 0 100 5 10
    240605182858_yeqa AACACTTATGGTCCGATCAGAGCCTATCCCAG(+CAGTGT)GATCCAAGGGATGCATCCTAGTCCAGTGACC NGG 10 8 17 8 24 0 100 5 10