Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182736_5e7p ATGGAGGTAGGGGCGGTCAGATGAGGTGGGTG(+CAGTGT)GTACCCAGGGGCACATCCTTGGCTCTGCCCC NGG 10 8 17 8 24 0 100 5 10
    240605182735_qvzt TGTAGGTCCCCTGCCCTATGTTCTGAAGAATG(+CAGTGT)GATCCTAAACTCATGTGGCCTCTGATATTTA NGG 10 8 17 8 24 0 100 5 10
    240605182733_558d TCTTGTAAATATCAGAGGCCACATGAGTTTAG(+CAGTGT)GATCCATTCTTCAGAACATAGGGCAGGGGAC NGG 10 8 17 8 24 0 100 5 10
    240605182732_4u1x CATCCCTAGAGGCCCAGAATGGGGGTTGCTGG(+CAGTGT)GTACCCCATGCCCTGAGGGTTCCAGCACTGC NGG 10 8 17 8 24 0 100 5 10
    240605182730_z2im TGGCAGCAGTGCTGGAACCCTCAGGGCATGGG(+CAGTGT)GTACCCAGCAACCCCCATTCTGGGCCTCTAG NGG 10 8 17 8 24 0 100 5 10
    240605182727_n7me ATCAGTATCCAGAAGCAGAGTGGTTGCCCCAG(+CAGTGT)GATCCCACCTCACGTGTGATGCTACCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605182725_e3gt AGGAGTGCTGGGTAGCATCACACGTGAGGTGG(+CAGTGT)GATCCTGGGGCAACCACTCTGCTTCTGGATA NGG 10 8 17 8 24 0 100 5 10
    240605182723_6g6y ATGCCCAGGAAAAATCCAGATCTCCATAGCTG(+CAGTGT)GATCCTGGGTGGCTGGCTGGCGCAGGCATCT NGG 10 8 17 8 24 0 100 5 10
    240605182720_b1ax GCCATAGATGCCTGCGCCAGCCAGCCACCCAG(+CAGTGT)GATCCAGCTATGGAGATCTGGATTTTTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605182719_lp8p GGGAATCTGGTCCCTCCTTGTGAGTTCCTGAG(+CAGTGT)GATCCAACTCAGTTAGGCTTGGTAGCGAGGG NGG 10 8 17 8 24 0 100 5 10