Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182645_4qeg GCTATCTTCAGATTCACCCAAAGAAGGCATTG(+CAGTGT)GATCCCATTACAGATGATTGCAAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605182644_wwuu TTTTTTTTTTTTTGGTGTGTGTATTGAAATTG(+CAGTGT)GATCCATGATTTTGCATATGCTAGGCAAGTA NGG 10 8 17 8 24 0 100 5 10
    240605182643_5z8p AGTACTACTTGCCTAGCATATGCAAAATCATG(+CAGTGT)GATCCAATTTCAATACACACACCAAAAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605182640_u4yn ACTAGGCCTGATAATCTTAGTTCAATCCCTGG(+CAGTGT)GATCCATACAGTTGTGGGAGAGAAGCAACTT NGG 10 8 17 8 24 0 100 5 10
    240605182638_btai TACAGAAGTTGCTTCTCTCCCACAACTGTATG(+CAGTGT)GATCCCAGGGATTGAACTAAGATTATCAGGC NGG 10 8 17 8 24 0 100 5 10
    240605182637_no2q GAAGACCCAGGTTCATTGCCAGCACCCACATG(+CAGTGT)GTACCTCACACCCACCTATTACTACAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240605182636_0s8p CCTCTGGAACTGTAGTAATAGGTGGGTGTGAG(+CAGTGT)GTACCATGTGGGTGCTGGCAATGAACCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605182635_mwgw TGTAGCTGAAAACCCAAGCCATGGCCTCTCAG(+CAGTGT)GATCCGTGTGCAATCTCAGGGCCCTGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605182634_g6a3 GGCAAGAGCCCAGGGCCCTGAGATTGCACACG(+CAGTGT)GATCCTGAGAGGCCATGGCTTGGGTTTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605182632_g3tk CATGCTTGCTTGGTCCAATTACTTGTGTGTTG(+CAGTGT)GATCCAAGATGTATTTTAAACAAATATTGGC NGG 10 8 17 8 24 0 100 5 10