Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182549_x992 GTATTTTATTAAACAGGCAAGCAGGGCTTCAG(+CAGTGT)GTACCTGTTTGATCTTTGAGTCTGGAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240605182547_vz7e GGTAGAGTGCCCCCAAGTGGCAGGTAGCAGTG(+CAGTGT)GTACCAATCATATGACCCAGATAAGCCCTCC NGG 10 8 17 8 24 0 100 5 10
    240605182546_575l TAGAGGGAGGGCTTATCTGGGTCATATGATTG(+CAGTGT)GTACCACTGCTACCTGCCACTTGGGGGCACT NGG 10 8 17 8 24 0 100 5 10
    240605182544_a2cr GTGACAGTCTCTCCCTTCCCTGCTTGCCGTAG(+CAGTGT)GATCCGTGCAAATGTAGTTTGAAGATGCAAC NGG 10 8 17 8 24 0 100 5 10
    240605182543_k0ac TGGTGGTTGCATCTTCAAACTACATTTGCACG(+CAGTGT)GATCCTACGGCAAGCAGGGAAGGGAGAGACT NGG 10 8 17 8 24 0 100 5 10
    240605182542_99r0 CATTTGCTTTCCTTTGCCCTTAAATAAATATG(+CAGTGT)GTACCAGAAAGCCCACATTGTGAGTTGTCTT NGG 10 8 17 8 24 0 100 5 10
    240605182540_a8mm TGCATAAGACAACTCACAATGTGGGCTTTCTG(+CAGTGT)GTACCATATTTATTTAAGGGCAAAGGAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605182539_j93j ACCAAATATACAGCATCAATAAAATGTACACG(+CAGTGT)GTACCATGAAAAATTCGAGAACTCAAGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605182538_gucl GAAAAGCAGCTTGAGTTCTCGAATTTTTCATG(+CAGTGT)GTACCGTGTACATTTTATTGATGCTGTATAT NGG 10 8 17 8 24 0 100 5 10
    240605182537_inua ACTGCTGGTGTATCTGTGAAGTGTTTGCAGTG(+CAGTGT)GATCCATCTGCCGCTGCCTGCTAACATATGA NGG 10 8 17 8 24 0 100 5 10