Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182502_5ins GTTGACCTTGGGGAGGGGGAAACAATGAATAG(+CAGTGT)GATCCTTTAGAGTCTGGAGATGATTCTAGGT NGG 10 8 17 8 24 0 100 5 10
    240605182500_4wzu AAGTGACTCTTCGGGTGGCTTTCCCTCTCAGG(+CAGTGT)GTACCTAGAGGTGTGAATTTGAAGCCATGCT NGG 10 8 17 8 24 0 100 5 10
    240605182459_5060 TTCCCAGCATGGCTTCAAATTCACACCTCTAG(+CAGTGT)GTACCCTGAGAGGGAAAGCCACCCGAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240605182457_j9h2 TATTCACACTCCTAATCAAACGTGCACTATGG(+CAGTGT)GTACCTAGTTTTTCTCTTAAGAACATACATG NGG 10 8 17 8 24 0 100 5 10
    240605182456_wqzj ACTTACATGTATGTTCTTAAGAGAAAAACTAG(+CAGTGT)GTACCCATAGTGCACGTTTGATTAGGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605182455_s2fg TTATTCAAATTTTGATTATTTTAAAAATAAAG(+CAGTGT)GATCCATTCTTACCGAAAGGGACACTACATC NGG 10 8 17 8 24 0 100 5 10
    240605182454_oppk TTAAAGATGTAGTGTCCCTTTCGGTAAGAATG(+CAGTGT)GATCCTTTATTTTTAAAATAATCAAAATTTG NGG 10 8 17 8 24 0 100 5 10
    240605182452_ulyq CTCCACTTCCCATCTGACCGCTGATGTGCTGG(+CAGTGT)GATCCCAGAGCATACTCCTGAGCCTGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605182450_xrv6 GTCATAAAGCCAGGCTCAGGAGTATGCTCTGG(+CAGTGT)GATCCCAGCACATCAGCGGTCAGATGGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605182449_9yus TATGTTAATATGATGAAATAAAATCAGAACTG(+CAGTGT)GTACCTATTTGTACGTAAGTGTGAGACGTGG NGG 10 8 17 8 24 0 100 5 10