Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182536_wk0p TTAGTTCATATGTTAGCAGGCAGCGGCAGATG(+CAGTGT)GATCCACTGCAAACACTTCACAGATACACCA NGG 10 8 17 8 24 0 100 5 10
    240605182534_lfzz GTTCCAGGAGCCTCCTCATGTTTGCCTGCTGG(+CAGTGT)GATCCCTGCACAAGGTGTTTCTTTACAATAG NGG 10 8 17 8 24 0 100 5 10
    240605182532_e1ss CTGGTCTATTGTAAAGAAACACCTTGTGCAGG(+CAGTGT)GATCCCAGCAGGCAAACATGAGGAGGCTCCT NGG 10 8 17 8 24 0 100 5 10
    240605182530_8vnz AATACTACCATAAATAACTAGCACCCACCAAG(+CAGTGT)GATCCCAAGTTCCTGTCTTTTCCAAATGGCT NGG 10 8 17 8 24 0 100 5 10
    240605182528_8luz ACCCTAGCCATTTGGAAAAGACAGGAACTTGG(+CAGTGT)GATCCTTGGTGGGTGCTAGTTATTTATGGTA NGG 10 8 17 8 24 0 100 5 10
    240605182527_bmd2 GTCAGGAGAACACTTGAAAGGCTTTCTTTGTG(+CAGTGT)GTACCTACGGGGTGAAATCACAGCCTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240605182525_x2jj CTCTCTTGACAGGCTGTGATTTCACCCCGTAG(+CAGTGT)GTACCACAAAGAAAGCCTTTCAAGTGTTCTC NGG 10 8 17 8 24 0 100 5 10
    240605182523_f3dy ATTTGAAATGGTTGATAAAATCCTCAAGCTAG(+CAGTGT)GATCCCCCAGAAGGTGTTTGATTGCTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605182520_t6h7 AAGGGAACAAAGCAATCAAACACCTTCTGGGG(+CAGTGT)GATCCTAGCTTGAGGATTTTATCAACCATTT NGG 10 8 17 8 24 0 100 5 10
    240605182519_7tdl ATCATCTTACCATTCTGATCCTTCATGCTCTG(+CAGTGT)GTACCCGAGCTCGGAGCGCCCCATCCGTCTA NGG 10 8 17 8 24 0 100 5 10