Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182517_b462 ACAGGTAGACGGATGGGGCGCTCCGAGCTCGG(+CAGTGT)GTACCAGAGCATGAAGGATCAGAATGGTAAG NGG 10 8 17 8 24 0 100 5 10
    240605182516_8aqd AACATAATTAGAAAGTCTCCTTCTGGCTGATG(+CAGTGT)GTACCACTGGTCTCTCACCACACTAAGGTTT NGG 10 8 17 8 24 0 100 5 10
    240605182514_k3h6 TGGCAAAACCTTAGTGTGGTGAGAGACCAGTG(+CAGTGT)GTACCATCAGCCAGAAGGAGACTTTCTAATT NGG 10 8 17 8 24 0 100 5 10
    240605182512_962o GCTAGTCCTATACTTTTACTTAATCTTCTCTG(+CAGTGT)GTACCAGGTAATATTCTCTTTCTTTCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605182510_ub8v CTGGGCAGGAGAAAGAAAGAGAATATTACCTG(+CAGTGT)GTACCAGAGAAGATTAAGTAAAAGTATAGGA NGG 10 8 17 8 24 0 100 5 10
    240605182509_lqtv TTAGAAACAGTCTCAGACTTTTTTACAGAAAG(+CAGTGT)GTACCGATAAACAATACACCCTGAGTCTTCA NGG 10 8 17 8 24 0 100 5 10
    240605182508_kc74 AGTCTTGAAGACTCAGGGTGTATTGTTTATCG(+CAGTGT)GTACCTTTCTGTAAAAAAGTCTGAGACTGTT NGG 10 8 17 8 24 0 100 5 10
    240605182506_re5p CGCTTTTTCTTCTTCTTTTAATTTCTCTATGG(+CAGTGT)GATCCTGGAGTTCTGGTTCCTTACTGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605182504_r2t3 GGGTTGCCTTCAGTAAGGAACCAGAACTCCAG(+CAGTGT)GATCCCATAGAGAAATTAAAAGAAGAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605182503_0bm2 AAATAACCTAGAATCATCTCCAGACTCTAAAG(+CAGTGT)GATCCTATTCATTGTTTCCCCCTCCCCAAGG NGG 10 8 17 8 24 0 100 5 10