Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182416_dx71 AGACAAAACTGTTTTGTATGTGACTAGCTGGG(+CAGTGT)GTACCCTGGGGGGATGATTCGATGGGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605182415_gi22 ACAAAGACAAGGACCAGAAGCAAATACTGGTG(+CAGTGT)GATCCAGATGTTTGAAATCTTTGTATCAAAA NGG 10 8 17 8 24 0 100 5 10
    240605182413_xkcm ACACGTTTTGATACAAAGATTTCAAACATCTG(+CAGTGT)GATCCACCAGTATTTGCTTCTGGTCCTTGTC NGG 10 8 17 8 24 0 100 5 10
    240605182411_fodh GCTCAACAATACAGAGAATATGTGAACCTGAG(+CAGTGT)GTACCCTGACTCAAATTTCACAACCACAATG NGG 10 8 17 8 24 0 100 5 10
    240605182409_xsmw GGTTTCATTGTGGTTGTGAAATTTGAGTCAGG(+CAGTGT)GTACCTCAGGTTCACATATTCTCTGTATTGT NGG 10 8 17 8 24 0 100 5 10
    240605182408_vqyl AAGACATATACATATATGTTAATCAAATATTG(+CAGTGT)GTACCAGAGTACACACTGTGTTTGCATGATT NGG 10 8 17 8 24 0 100 5 10
    240605182407_6ee1 GAGAAAATCATGCAAACACAGTGTGTACTCTG(+CAGTGT)GTACCAATATTTGATTAACATATATGTATAT NGG 10 8 17 8 24 0 100 5 10
    240605182405_oaxu CCAGGTCAGTTGTAGGAATTCTTGAGTATCAG(+CAGTGT)GATCCATCGATGTGTAGACAAAACTGTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605182404_aix8 CACTCTAAAACAGTTTTGTCTACACATCGATG(+CAGTGT)GATCCTGATACTCAAGAATTCCTACAACTGA NGG 10 8 17 8 24 0 100 5 10
    240605182402_1whn TGAACCTGAGACCCTCCTGTGTCAACCCTTCG(+CAGTGT)GTACCTGGGATTACAGATGCTTCTCAGGATC NGG 10 8 17 8 24 0 100 5 10