Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182310_c28f TGTTTCAGTTAAGCTCAAAGGCCACTCATCAG(+CAGTGT)GTACCCAAGTTGCCCAAGAACTCAAGGGAGT NGG 10 8 17 8 24 0 100 5 10
    240605182308_lwvc ATGTGGTGCCTGTTCAAAGTCCTGCATAAGGG(+CAGTGT)GATCCCTGTACACTCGAACCGTTATTATTTG NGG 10 8 17 8 24 0 100 5 10
    240605182306_i3c1 ATCATCAAATAATAACGGTTCGAGTGTACAGG(+CAGTGT)GATCCCCTTATGCAGGACTTTGAACAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240605182305_5xh6 CTGTGTGTAGCATTGAATGGCTTCAGGGTTTG(+CAGTGT)GATCCCGTTGAGTGGGGCAAGGTAGGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605182303_2cgf ACTTGGGCCTCCTACCTTGCCCCACTCAACGG(+CAGTGT)GATCCAAACCCTGAAGCCATTCAATGCTACA NGG 10 8 17 8 24 0 100 5 10
    240605182300_n4fz CAGCCAGGCTGGGGGTACATGTAGCACCCCTG(+CAGTGT)GATCCCATCTTGTTGCTAGCAGGGTAAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605182258_pigv AGGGGTCACTTACCCTGCTAGCAACAAGATGG(+CAGTGT)GATCCAGGGGTGCTACATGTACCCCCAGCCT NGG 10 8 17 8 24 0 100 5 10
    240605182257_vppz GCCTGCTGTTCATCTGACAAGCAAGAGCAGTG(+CAGTGT)GATCCATTTCTAAGGCTGTGTACAGGAAGAC NGG 10 8 17 8 24 0 100 5 10
    240605182256_5je4 CCACTGTCTTCCTGTACACAGCCTTAGAAATG(+CAGTGT)GATCCACTGCTCTTGCTTGTCAGATGAACAG NGG 10 8 17 8 24 0 100 5 10
    240605182254_hnxk AGTCCTCCCCTGACTCTACCTAGCTAATCCTG(+CAGTGT)GATCCCATTTTACTTAGGAAAACAAAAACAA NGG 10 8 17 8 24 0 100 5 10