Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182221_jl7u AAAAAAAAAAATCAAAATAAATTGAACCCCCG(+CAGTGT)GATCCCTCAACTACAAATGTCAATAATGAAT NGG 10 8 17 8 24 0 100 5 10
    240605182220_h8yk TGGCCACCACCTCCCCCGGACTCTACCTCAAG(+CAGTGT)GATCCACCTCAAACCAAGCCTGACCCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605182218_2xao GGTGATGCCTGGGTCAGGCTTGGTTTGAGGTG(+CAGTGT)GATCCTTGAGGTAGAGTCCGGGGGAGGTGGT NGG 10 8 17 8 24 0 100 5 10
    240605182217_otdz AAGGAGGCGACGCAGAGGCACTGGGGTACAGG(+CAGTGT)GTACCAAATGAAAGAGAGAGAGTCTTGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605182215_f2u9 CTCCATTCTCAAGACTCTCTCTCTTTCATTTG(+CAGTGT)GTACCCTGTACCCCAGTGCCTCTGCGTCGCC NGG 10 8 17 8 24 0 100 5 10
    240605182212_hx0g AAAAATGGAATGAGCCACCGGCAGGTGCCCTG(+CAGTGT)GTACCAGAAGGACTGACTGCCGTGAGAGACA NGG 10 8 17 8 24 0 100 5 10
    240605182211_khx9 GATAGTGTCTCTCACGGCAGTCAGTCCTTCTG(+CAGTGT)GTACCAGGGCACCTGCCGGTGGCTCATTCCA NGG 10 8 17 8 24 0 100 5 10
    240605182210_pytx TGCCCTTCTGAGACCAACAGGGACAAGAGCAG(+CAGTGT)GATCCCAGTGTGTGGCCCAGCAAGCTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605182208_lycw GAGTCTCCCCAGCTTGCTGGGCCACACACTGG(+CAGTGT)GATCCTGCTCTTGTCCCTGTTGGTCTCAGAA NGG 10 8 17 8 24 0 100 5 10
    240605182207_jqtb ACCCGCCGGGCACCTGGCTTTGTCCCATGCGG(+CAGTGT)GATCCTATCATGACACTAGAAGAAGCCCCCG NGG 10 8 17 8 24 0 100 5 10