Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182133_1v08 CCATCTGGTGAGGGGTGAGCAGGGGAGGAGAG(+CAGTGT)GTACCACTGGAAGATGGTCAGCTGATGCAGA NGG 10 8 17 8 24 0 100 5 10
    240605182131_5ypg CAACTCTCTAGGCCTTACTTTGGGGAGCCTGG(+CAGTGT)GATCCTCTTCCAATTTCTCCATTTGGCCCCA NGG 10 8 17 8 24 0 100 5 10
    240605182130_nuu4 AGAGTTGGGGCCAAATGGAGAAATTGGAAGAG(+CAGTGT)GATCCCAGGCTCCCCAAAGTAAGGCCTAGAG NGG 10 8 17 8 24 0 100 5 10
    240605182128_r7kg ACCACCCTGGAATAGGGGCATCAAGATAGGAG(+CAGTGT)GTACCCAGTTCAGAAGGTGAGTCCCAAATAC NGG 10 8 17 8 24 0 100 5 10
    240605182127_ayjw AGGGGGTATTTGGGACTCACCTTCTGAACTGG(+CAGTGT)GTACCTCCTATCTTGATGCCCCTATTCCAGG NGG 10 8 17 8 24 0 100 5 10
    240605182125_hnkl GGTCACTGCAGGGGAATGGTCTCAAGCTGCAG(+CAGTGT)GATCCTAACCTGGAAGAGGAAGCCATGGTCT NGG 10 8 17 8 24 0 100 5 10
    240605182124_cgos TGAACAGACCATGGCTTCCTCTTCCAGGTTAG(+CAGTGT)GATCCTGCAGCTTGAGACCATTCCCCTGCAG NGG 10 8 17 8 24 0 100 5 10
    240605182123_dt65 ATCGATCCAGACGAGGTCAGGGCTGTGCTGTG(+CAGTGT)GATCCATCACACAAGCGTCTGTCCATCCCAT NGG 10 8 17 8 24 0 100 5 10
    240605182122_3xaq GAGAGATGGGATGGACAGACGCTTGTGTGATG(+CAGTGT)GATCCACAGCACAGCCCTGACCTCGTCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605182120_f4pa AAGCCTCTGCACTCTTGCCATCCCGTGTCAGG(+CAGTGT)GATCCAGTGTGGGTGGGCGGCTGAGGACCTC NGG 10 8 17 8 24 0 100 5 10