Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182026_omon CTGCTTCAATAGCATGTCCCAAGGAGTCCGGG(+CAGTGT)GATCCAGGATCGATCAGAACACACCATGGGT NGG 10 8 17 8 24 0 100 5 10
    240605182025_miip AAGCCCCTCTTGTTCCTCCATCACTAAAAGAG(+CAGTGT)GATCCCTGAAATCAGACCAAAGCCACACATC NGG 10 8 17 8 24 0 100 5 10
    240605182023_91td TATTTGATGTGTGGCTTTGGTCTGATTTCAGG(+CAGTGT)GATCCTCTTTTAGTGATGGAGGAACAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240605182022_56t1 CACAGAGAAGCCTGGCAGAATGGAACCCAGTG(+CAGTGT)GTACCTGCCCCACAAGAATTCTAGGACATTC NGG 10 8 17 8 24 0 100 5 10
    240605182020_fue1 GGTGTGAATGTCCTAGAATTCTTGTGGGGCAG(+CAGTGT)GTACCACTGGGTTCCATTCTGCCAGGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605182018_4bg9 GTTGGCCTTCTCACTTTGATAGTTTAACTCAG(+CAGTGT)GTACCCAGTCTTCACTCCATCCCCAGGTGCT NGG 10 8 17 8 24 0 100 5 10
    240605182016_h246 GAACAAGCACCTGGGGATGGAGTGAAGACTGG(+CAGTGT)GTACCTGAGTTAAACTATCAAAGTGAGAAGG NGG 10 8 17 8 24 0 100 5 10
    240605182015_b521 AACTTCAAAGCTTTATTCTGTCTGCCTGAGAG(+CAGTGT)GATCCATGGAAGATGATTCTCCCAAAAAGCA NGG 10 8 17 8 24 0 100 5 10
    240605182013_k03u TTTTCTGCTTTTTGGGAGAATCATCTTCCATG(+CAGTGT)GATCCTCTCAGGCAGACAGAATAAAGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605182012_4dko GAAGGCTTTCTACTCCTTAACAGGTACAGTTG(+CAGTGT)GATCCATCGCTGATGGTGTTTCTTTAGCCCC NGG 10 8 17 8 24 0 100 5 10