Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182100_jrw9 GATGTCTGAGACTGGAGTATTCTCTTGGCCGG(+CAGTGT)GATCCACTCACCGCTGTGCGGCATAGGATGT NGG 10 8 17 8 24 0 100 5 10
    240605182058_pl4g TTAGGCAAAGTTAATAGGGTATCTGGCATTGG(+CAGTGT)GATCCTACTGACTGCCATTCAGTAAGCCAAT NGG 10 8 17 8 24 0 100 5 10
    240605182057_4jgh TAACTATTGGCTTACTGAATGGCAGTCAGTAG(+CAGTGT)GATCCCAATGCCAGATACCCTATTAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240605182055_e85d TTCAGGGAAAAAGAAAACATGTTTTACCTAGG(+CAGTGT)GATCCTTCTGAACAAAATGGCTGTCTGTACT NGG 10 8 17 8 24 0 100 5 10
    240605182053_qqmn AAAAGAGTACAGACAGCCATTTTGTTCAGAAG(+CAGTGT)GATCCCTAGGTAAAACATGTTTTCTTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605182050_k1fg ATCAGGGGTTGGGTCCTGGACGACAACACAGG(+CAGTGT)GATCCTTTGAGACCCTCTTAAGTGGGCCAAA NGG 10 8 17 8 24 0 100 5 10
    240605182049_4wws CATACTTTGGCCCACTTAAGAGGGTCTCAAAG(+CAGTGT)GATCCCTGTGTTGTCGTCCAGGACCCAACCC NGG 10 8 17 8 24 0 100 5 10
    240605182048_xw59 CAGAGAGCACCGGCTGCTGTTCAGGATCACAG(+CAGTGT)GATCCTCTAGGCTGGGCCACAGTCTTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605182046_o7eg AAGAGGAAGGAAGACTGTGGCCCAGCCTAGAG(+CAGTGT)GATCCTGTGATCCTGAACAGCAGCCGGTGCT NGG 10 8 17 8 24 0 100 5 10
    240605182045_z7cu GAAAGAAACAGATACATGAGGCTTTCACTTAG(+CAGTGT)GATCCTCAGTATCTGCCTTCAACAACCCTTA NGG 10 8 17 8 24 0 100 5 10