Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182149_p66d GGAGGTCCCTGCTAAGAACGGGCAGGAGTGAG(+CAGTGT)GATCCACTTGAAACCGGGAGACCCCATTTAA NGG 10 8 17 8 24 0 100 5 10
    240605182148_1ogt GATGCTGCACTATTGAACAAGAAAACAGTAAG(+CAGTGT)GTACCACCAACACAACCCTCTTGTCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605182147_fijl TTTTTTCCTTTGACAAGAGGGTTGTGTTGGTG(+CAGTGT)GTACCTTACTGTTTTCTTGTTCAATAGTGCA NGG 10 8 17 8 24 0 100 5 10
    240605182145_m61c GATGGACCTCACCCCTTTTCCACAGGTCACTG(+CAGTGT)GATCCCACTGTCACCCTCGGTGCTTACACAT NGG 10 8 17 8 24 0 100 5 10
    240605182144_l4ak CTGAAATGTGTAAGCACCGAGGGTGACAGTGG(+CAGTGT)GATCCAGTGACCTGTGGAAAAGGGGTGAGGT NGG 10 8 17 8 24 0 100 5 10
    240605182141_rkps AAATCACTGCCCCATACTCAAACGACTCCCAG(+CAGTGT)GTACCCATTACTAGAAGTTTCTAAGTCACAG NGG 10 8 17 8 24 0 100 5 10
    240605182140_jddx GGAGCCTGTGACTTAGAAACTTCTAGTAATGG(+CAGTGT)GTACCTGGGAGTCGTTTGAGTATGGGGCAGT NGG 10 8 17 8 24 0 100 5 10
    240605182138_kpet AAGCCACTTAAAGACCTATACACAACGTGATG(+CAGTGT)GTACCTTAGAATTCTGTAGTTGGGGAATTTC NGG 10 8 17 8 24 0 100 5 10
    240605182136_847v AATGTGAAATTCCCCAACTACAGAATTCTAAG(+CAGTGT)GTACCATCACGTTGTGTATAGGTCTTTAAGT NGG 10 8 17 8 24 0 100 5 10
    240605182134_z7zx CTGTCTCTGCATCAGCTGACCATCTTCCAGTG(+CAGTGT)GTACCTCTCCTCCCCTGCTCACCCCTCACCA NGG 10 8 17 8 24 0 100 5 10