Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182344_xvih ATCGCTCTGGGGTAGGGTTGGGGGCTCGGAAG(+CAGTGT)GATCCTTCACCAGCTAAGTGGTCGAGCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605182341_rr70 CTTGCCTGCTAGCTTCCCTCAGACGGCTTGGG(+CAGTGT)GTACCCTGAGCCACACAAGTGCCAGGATAGC NGG 10 8 17 8 24 0 100 5 10
    240605182340_i800 CGCCTGCTATCCTGGCACTTGTGTGGCTCAGG(+CAGTGT)GTACCCCAAGCCGTCTGAGGGAAGCTAGCAG NGG 10 8 17 8 24 0 100 5 10
    240605182338_c45y TTGGGGAGCTGCGAGGAAAGGGCAGTAAGCAG(+CAGTGT)GATCCTGGCACGGCAACCTGTATCTCTAGAA NGG 10 8 17 8 24 0 100 5 10
    240605182336_sggy TAGGCTTCTAGAGATACAGGTTGCCGTGCCAG(+CAGTGT)GATCCTGCTTACTGCCCTTTCCTCGCAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605182333_fk8l CCAGCAGGGAGTGGAGGACTCGGACTCTGGGG(+CAGTGT)GATCCTACTGCTTGCATAGGTCCTGGTGTGG NGG 10 8 17 8 24 0 100 5 10
    240605182332_5yai CTAAGCCACACCAGGACCTATGCAAGCAGTAG(+CAGTGT)GATCCCCCAGAGTCCGAGTCCTCCACTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605182330_w3qp TCTGCCTCGAACTAATTCATCTCACGCTGTTG(+CAGTGT)GTACCGAGTGTGAGGCTCAGCGACTGGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605182329_wd2j TGATTTTCTCCAGTCGCTGAGCCTCACACTCG(+CAGTGT)GTACCAACAGCGTGAGATGAATTAGTTCGAG NGG 10 8 17 8 24 0 100 5 10
    240605182328_ekf4 TCCCTTGCCTAAGGCAGAGCCGTTTCCTTCAG(+CAGTGT)GTACCACACAAGCAATTTGCAGACCTGACTC NGG 10 8 17 8 24 0 100 5 10