Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182432_5njo GGATGGACCATGTAGAGACTGCCATATCCAGG(+CAGTGT)GATCCACCCCATAATCAGCATCCAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240605182431_qmve CAAGGTTTGAGGGTATTTGAATAAAATTTTTG(+CAGTGT)GATCCAGCTGCAACATAAAGAGTGCCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605182430_3nsj ATGTGGGAAGGGCACTCTTTATGTTGCAGCTG(+CAGTGT)GATCCAAAAATTTTATTCAAATACCCTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605182428_fgib AGTGCCAGGCCTTGGGTCCTCCCCTTGAGATG(+CAGTGT)GATCCCACTTCAGGCCTGTCAGTGGACCTTC NGG 10 8 17 8 24 0 100 5 10
    240605182426_je8w GAAAAGAAGGTCCACTGACAGGCCTGAAGTGG(+CAGTGT)GATCCATCTCAAGGGGAGGACCCAAGGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605182425_dorj CAAGGTTTGAGGATATTTGAATAAAATTTTTG(+CAGTGT)GATCCAGCTGCAACATAAAGAGTGCCCTTCC NGG 10 8 17 8 24 0 100 5 10
    240605182424_4831 ATGTGGGAAGGGCACTCTTTATGTTGCAGCTG(+CAGTGT)GATCCAAAAATTTTATTCAAATATCCTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605182422_oq6j TGAAGCAATAGAAACATGCGATTTAGCTTTGG(+CAGTGT)GATCCTCTGAATGAGGGATTCCCCCAAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605182421_jphn TTATAGCCTTTGGGGGAATCCCTCATTCAGAG(+CAGTGT)GATCCCAAAGCTAAATCGCATGTTTCTATTG NGG 10 8 17 8 24 0 100 5 10
    240605182418_ce6y AGCCCCCTCCCCCATCGAATCATCCCCCCAGG(+CAGTGT)GTACCCCAGCTAGTCACATACAAAACAGTTT NGG 10 8 17 8 24 0 100 5 10