Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182631_92pi TCTATGCCAATATTTGTTTAAAATACATCTTG(+CAGTGT)GATCCAACACACAAGTAATTGGACCAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240605182630_ldrn AATTGTAACATAGGTAGTATGCCTTCACACTG(+CAGTGT)GATCCCGGGGGCATGGGTTTTGGAGATGTTA NGG 10 8 17 8 24 0 100 5 10
    240605182626_y2uq CAAAATAACATCTCCAAAACCCATGCCCCCGG(+CAGTGT)GATCCAGTGTGAAGGCATACTACCTATGTTA NGG 10 8 17 8 24 0 100 5 10
    240605182625_xy7a CACCACCTTGCTACGTGCATGTACCAGGCAAG(+CAGTGT)GTACCAAAGATAACAAATGAAAAACAAACCC NGG 10 8 17 8 24 0 100 5 10
    240605182624_xmlv ATCATGGGTTTGTTTTTCATTTGTTATCTTTG(+CAGTGT)GTACCTTGCCTGGTACATGCACGTAGCAAGG NGG 10 8 17 8 24 0 100 5 10
    240605182623_um6p AATCAGCTTGCTTTGTTGGCCTAGATATTTTG(+CAGTGT)GATCCTTTATTGAAATGAGGCAGTGAGATAG NGG 10 8 17 8 24 0 100 5 10
    240605182621_cnvi GGAAACTATCTCACTGCCTCATTTCAATAAAG(+CAGTGT)GATCCAAAATATCTAGGCCAACAAAGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240605182620_x7zq AGTGGAAATAATTTGCTGTCGTTTCAGACTTG(+CAGTGT)GATCCTACAAGCCTGTCCGTTCTGTAAGTGG NGG 10 8 17 8 24 0 100 5 10
    240605182619_p0pr CAGTACCACTTACAGAACGGACAGGCTTGTAG(+CAGTGT)GATCCAAGTCTGAAACGACAGCAAATTATTT NGG 10 8 17 8 24 0 100 5 10
    240605182618_04fd TGACAAGGGAGTCGGAAGCTAAGGAGGTGACG(+CAGTGT)GATCCTAATGCTTGTCAAATAAACCGATCGC NGG 10 8 17 8 24 0 100 5 10