Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182701_lckf ATAGAAGGCAGAGGAATTATTTGGGAAGAGGG(+CAGTGT)GATCCTCTCCAGGCTCTTAGCCAAACAAGGG NGG 10 8 17 8 24 0 100 5 10
    240605182700_c580 TGTGAGAATGGGGATTGATGAGGCCCAGCTTG(+CAGTGT)GTACCTGGAAAATGTGTATGGTAAGGAAAAT NGG 10 8 17 8 24 0 100 5 10
    240605182658_xwwr TGCACATTTTCCTTACCATACACATTTTCCAG(+CAGTGT)GTACCAAGCTGGGCCTCATCAATCCCCATTC NGG 10 8 17 8 24 0 100 5 10
    240605182657_0dot AGAGACTGCCTGGAGATGGACAGAGAAGCTGG(+CAGTGT)GATCCTTGGTGACCAGCCCCTCTGAAACACT NGG 10 8 17 8 24 0 100 5 10
    240605182655_gihw CCTAGAGTGTTTCAGAGGGGCTGGTCACCAAG(+CAGTGT)GATCCCAGCTTCTCTGTCCATCTCCAGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605182653_iaiq AACACTCCCTCAGTCCCAGAAGTTTCCCCACG(+CAGTGT)GATCCCCATTTGCATATAACCTCTTCCCATC NGG 10 8 17 8 24 0 100 5 10
    240605182651_hp4o GGTAGGATGGGAAGAGGTTATATGCAAATGGG(+CAGTGT)GATCCGTGGGGAAACTTCTGGGACTGAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605182649_u285 AGGAAATGGGAGGGAGATGAGAAAACAGGATG(+CAGTGT)GTACCAATCACCTGGGTAATCAACTGGTTGA NGG 10 8 17 8 24 0 100 5 10
    240605182648_s7wq CCTCTTCAACCAGTTGATTACCCAGGTGATTG(+CAGTGT)GTACCATCCTGTTTTCTCATCTCCCTCCCAT NGG 10 8 17 8 24 0 100 5 10
    240605182646_8ezl ACTACATGGTGGCTTGCAATCATCTGTAATGG(+CAGTGT)GATCCAATGCCTTCTTTGGGTGAATCTGAAG NGG 10 8 17 8 24 0 100 5 10