Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605182754_ov4q TCTTAGTTCTCATGAAGCACAGAATTTCTTTG(+CAGTGT)GATCCCAGGAGAAAAGTAAAAATGTCTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605182752_b9um ACACACATTTATATGCTATATCTGTATTAAGG(+CAGTGT)GATCCTCCAGGGTTGCCCTATTCTTCACCTC NGG 10 8 17 8 24 0 100 5 10
    240605182750_0qcg CACAGGAGGTGAAGAATAGGGCAACCCTGGAG(+CAGTGT)GATCCCTTAATACAGATATAGCATATAAATG NGG 10 8 17 8 24 0 100 5 10
    240605182749_30te GGAGTATGCCGAAAGGATCGGAGTCTAAACTG(+CAGTGT)GTACCCTAGCATTTCAAAGGTCCTCAACAGA NGG 10 8 17 8 24 0 100 5 10
    240605182747_b8ql AAGTATCTGTTGAGGACCTTTGAAATGCTAGG(+CAGTGT)GTACCAGTTTAGACTCCGATCCTTTCGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605182745_86bb AGCAAATTGTCCAGAAGACCAATGAGGCCAAG(+CAGTGT)GTACCCCTGAGATCCGGAAGACCCGGACATA NGG 10 8 17 8 24 0 100 5 10
    240605182743_0vol TCCTTTATGTCCGGGTCTTCCGGATCTCAGGG(+CAGTGT)GTACCTTGGCCTCATTGGTCTTCTGGACAAT NGG 10 8 17 8 24 0 100 5 10
    240605182742_6q54 CGAGTGTGGTCGCGGAGGGTGGAGGGGGATGG(+CAGTGT)GATCCTCCTTCTCCCATAGAGACCAGAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605182740_psac ATATCTAGCTCTGGTCTCTATGGGAGAAGGAG(+CAGTGT)GATCCCATCCCCCTCCACCCTCCGCGACCAC NGG 10 8 17 8 24 0 100 5 10
    240605182738_n0pg CCCGTGGGGCAGAGCCAAGGATGTGCCCCTGG(+CAGTGT)GTACCACCCACCTCATCTGACCGCCCCTACC NGG 10 8 17 8 24 0 100 5 10