Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180819_e9yg ACTGTGGAGAAGGACTTAAAGGAAGGTGACTG(+CAGTGT)GATCCTCTCTAAGGGTCACCAGCATTTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605180817_hstv GAAGCCTTCATGAGTCTTCCACTCTCTTCTGG(+CAGTGT)GTACCGACGCAGAGATGGCATGTCACAGGAT NGG 10 8 17 8 24 0 100 5 10
    240605180816_o6nb CCGGAATCCTGTGACATGCCATCTCTGCGTCG(+CAGTGT)GTACCCAGAAGAGAGTGGAAGACTCATGAAG NGG 10 8 17 8 24 0 100 5 10
    240605180814_jcw3 GTTACCTCACAGACCACCACAGCCCACTCTGG(+CAGTGT)GTACCATCATCCCTGTCTCTGCCATTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605180813_7s65 CTAGGGCAGGAATGGCAGAGACAGGGATGATG(+CAGTGT)GTACCCAGAGTGGGCTGTGGTGGTCTGTGAG NGG 10 8 17 8 24 0 100 5 10
    240605180812_nwnd TTCTGCCAGTGTGTGTGACTATGTGAGTGTTG(+CAGTGT)GATCCCCTGACACTGGAGTTACAGACAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605180810_6bi9 GCTTACAACTGTCTGTAACTCCAGTGTCAGGG(+CAGTGT)GATCCAACACTCACATAGTCACACACACTGG NGG 10 8 17 8 24 0 100 5 10
    240605180809_60pr GAATGTAAACAGTTTTATTCAGAAACAGATAG(+CAGTGT)GTACCTGTTTGCATTACAGAATATAAAACTT NGG 10 8 17 8 24 0 100 5 10
    240605180807_kysl AAACCAAGTTTTATATTCTGTAATGCAAACAG(+CAGTGT)GTACCTATCTGTTTCTGAATAAAACTGTTTA NGG 10 8 17 8 24 0 100 5 10
    240605180806_cl13 GGAGAAGAGTGGTTTGCCTTCTGAGGTATGAG(+CAGTGT)GATCCCTGGTCCAATCAGCAGGGTGAGGTTT NGG 10 8 17 8 24 0 100 5 10