Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180735_l6ej TGCTCTCTTTTAATGCAGCTTTGAAGAAGATG(+CAGTGT)GTACCAAAGGTTAAGACGGCCCCCTGATGGG NGG 10 8 17 8 24 0 100 5 10
    240605180732_zkrg TGGGCTAGGATCACTTGCCACAGAGCCCCAGG(+CAGTGT)GATCCACCTGTCTCTGTGTCCCCAGCACTGG NGG 10 8 17 8 24 0 100 5 10
    240605180730_8cyv TAACCCCAGTGCTGGGGACACAGAGACAGGTG(+CAGTGT)GATCCCTGGGGCTCTGTGGCAAGTGATCCTA NGG 10 8 17 8 24 0 100 5 10
    240605180727_eixo AGGTGATATTAGCACAAGTTACAATTCCCTGG(+CAGTGT)GTACCAAAACCTATACATATTCTTTGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240605180726_meje TTTTGAAACCCAAAGAATATGTATAGGTTTTG(+CAGTGT)GTACCCAGGGAATTGTAACTTGTGCTAATAT NGG 10 8 17 8 24 0 100 5 10
    240605180725_webe TCTACAGCAAGCTCAGAGGTAGCACAAGAGAG(+CAGTGT)GATCCATCTGGTAACACCATAAATCATGCAA NGG 10 8 17 8 24 0 100 5 10
    240605180723_95db CAATTTTGCATGATTTATGGTGTTACCAGATG(+CAGTGT)GATCCTCTCTTGTGCTACCTCTGAGCTTGCT NGG 10 8 17 8 24 0 100 5 10
    240605180722_iw1j ATCATAATTCTTTTTAATTTTAAAATAGTCAG(+CAGTGT)GATCCAGTTTGAAAATATGCCACGTTTAAAA NGG 10 8 17 8 24 0 100 5 10
    240605180721_6mws TCTCTTTTTAAACGTGGCATATTTTCAAACTG(+CAGTGT)GATCCTGACTATTTTAAAATTAAAAAGAATT NGG 10 8 17 8 24 0 100 5 10
    240605180720_0mbs TGGTGTTGGACGTCGGAAGTCCTCCTGTAGTG(+CAGTGT)GATCCAAAACTCACCTCCTTATGAAAGACAA NGG 10 8 17 8 24 0 100 5 10