Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180704_4izm CTAAAAGGAAGCAGTGTTTTGACTGCATAGGG(+CAGTGT)GTACCCAGAGGGTTTTGAGCAGATAAATTAC NGG 10 8 17 8 24 0 100 5 10
    240605180703_h82h TCATGGATGGTACTCTGGTTTTATTTGGAATG(+CAGTGT)GTACCTGATTTTCACTCACCATTATCTCATT NGG 10 8 17 8 24 0 100 5 10
    240605180701_ncnr GGCAGAATGAGATAATGGTGAGTGAAAATCAG(+CAGTGT)GTACCATTCCAAATAAAACCAGAGTACCATC NGG 10 8 17 8 24 0 100 5 10
    240605180700_h5d3 CATGGCAGGTGGCTCTAAGGACACCTAGCATG(+CAGTGT)GTACCCATTTGATTAAGAGAAGAAGATGCCT NGG 10 8 17 8 24 0 100 5 10
    240605180658_6frh CAGGAAGGCATCTTCTTCTCTTAATCAAATGG(+CAGTGT)GTACCATGCTAGGTGTCCTTAGAGCCACCTG NGG 10 8 17 8 24 0 100 5 10
    240605180656_yk5h TTAGGCTGTTTGGCAGATACAACAGAGTCCTG(+CAGTGT)GATCCTCCTTGCCTCCTTCAGAAGCTCCGTA NGG 10 8 17 8 24 0 100 5 10
    240605180654_x1th ACGCCTACGGAGCTTCTGAAGGAGGCAAGGAG(+CAGTGT)GATCCAGGACTCTGTTGTATCTGCCAAACAG NGG 10 8 17 8 24 0 100 5 10
    240605180652_xq24 ACTGTAAGAATAACAGACACCCCTTTTTGCTG(+CAGTGT)GTACCACCCTCCTCGAGTCCCCATGGTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605180650_edts AGGGTGCCAACCATGGGGACTCGAGGAGGGTG(+CAGTGT)GTACCAGCAAAAAGGGGTGTCTGTTATTCTT NGG 10 8 17 8 24 0 100 5 10
    240605180649_sq8g TGTCTACACGTCACTACTTACAATTTTAACTG(+CAGTGT)GTACCAAGTCTGTGCCCAGAGGCAACAAGCT NGG 10 8 17 8 24 0 100 5 10