Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180648_ih9e GGATTAGCTTGTTGCCTCTGGGCACAGACTTG(+CAGTGT)GTACCAGTTAAAATTGTAAGTAGTGACGTGT NGG 10 8 17 8 24 0 100 5 10
    240605180647_9vrs CTTCATACAGCAGTCATCTGCTCGTATGTTTG(+CAGTGT)GATCCCCTTTTAAACATTCAACCCTGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240605180645_3kek GATGGACCTCCAGGGTTGAATGTTTAAAAGGG(+CAGTGT)GATCCAAACATACGAGCAGATGACTGCTGTA NGG 10 8 17 8 24 0 100 5 10
    240605180643_oxl5 ACGATTCCCATTCTCTGTCTGTCTCGTATATG(+CAGTGT)GTACCATCTTCCAGTCACAGATCCTGACTCT NGG 10 8 17 8 24 0 100 5 10
    240605180642_z09o AGATGAGAGTCAGGATCTGTGACTGGAAGATG(+CAGTGT)GTACCATATACGAGACAGACAGAGAATGGGA NGG 10 8 17 8 24 0 100 5 10
    240605180641_yjyw TCTTCTGAAGTGTATGCTAAGTGTGTGTGGCG(+CAGTGT)GTACCTTCAGAGTCCAGAGGAAGGTATCAGA NGG 10 8 17 8 24 0 100 5 10
    240605180640_olt6 CAGGATCTGATACCTTCCTCTGGACTCTGAAG(+CAGTGT)GTACCGCCACACACACTTAGCATACACTTCA NGG 10 8 17 8 24 0 100 5 10
    240605180638_io2t CATACACTATTCCCGAGTTAGTCTGGTGTATG(+CAGTGT)GATCCACGTGCTCTCTTTTAGTATTATCTTA NGG 10 8 17 8 24 0 100 5 10
    240605180637_rug0 TATAATAAGATAATACTAAAAGAGAGCACGTG(+CAGTGT)GATCCATACACCAGACTAACTCGGGAATAGT NGG 10 8 17 8 24 0 100 5 10
    240605180635_7dqg TGCCCCATAGACCCGTTCTACACAGACAATGG(+CAGTGT)GATCCAGAGGGTCTGCGGCAAGAAGGTCCAA NGG 10 8 17 8 24 0 100 5 10