Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180849_rgrp CCCACGAAAAAAAGGTGTAGTTTCTGAAGAGG(+CAGTGT)GTACCACAATTTCTTAATTGCAGAGGAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605180847_xvfq CTGGGACCTCCCATGTGTCTTTATTCCCAATG(+CAGTGT)GTACCCAACTTGTCTCCTTCTACCTTGTTCT NGG 10 8 17 8 24 0 100 5 10
    240605180845_k8wh ACCAGAGAACAAGGTAGAAGGAGACAAGTTGG(+CAGTGT)GTACCATTGGGAATAAAGACACATGGGAGGT NGG 10 8 17 8 24 0 100 5 10
    240605180844_srb8 GAGGTCAACTACGAAGTGGCTGTTTCGCATTG(+CAGTGT)GTACCCAGCTGTGCCTCCACTATTTGCACAC NGG 10 8 17 8 24 0 100 5 10
    240605180843_yrsf AAGATGTGTGCAAATAGTGGAGGCACAGCTGG(+CAGTGT)GTACCAATGCGAAACAGCCACTTCGTAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605180842_574p TTCCACACAGAATACCACATTCTTACACAAAG(+CAGTGT)GTACCCACTTCACTTTGGTCTGCTGGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240605180840_qz5g TGTGAGCCCACCAGCAGACCAAAGTGAAGTGG(+CAGTGT)GTACCTTTGTGTAAGAATGTGGTATTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605180837_m2c1 ATCCCTCCCCACCCACCCACCCGTCCCCGCCG(+CAGTGT)GATCCGTAGTAAAGAGCCTGGCCTAAATATT NGG 10 8 17 8 24 0 100 5 10
    240605180836_u463 GGAGTAATATTTAGGCCAGGCTCTTTACTACG(+CAGTGT)GATCCGGCGGGGACGGGTGGGTGGGTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240605180834_9osd CCTATCTGCCAGTTCAGGCTCCTTCACACAGG(+CAGTGT)GATCCCAGAGAGGCTCCCAGGTTTCTCCTCC NGG 10 8 17 8 24 0 100 5 10