Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180933_jgnb TAGGCAGTCCTGAAACAAAGTGATAGGCACTG(+CAGTGT)GTACCAGGAATGTTGCCTCAGCCAGAGAACT NGG 10 8 17 8 24 0 100 5 10
    240605180931_2awf GTGTGTGTGTGTGTGTGTGTGTGTACTCTAGG(+CAGTGT)GTACCTCAGTATGTCCTGAAAGTTCAACAGA NGG 10 8 17 8 24 0 100 5 10
    240605180930_si8i AAAACTCTGTTGAACTTTCAGGACATACTGAG(+CAGTGT)GTACCCTAGAGTACACACACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240605180929_228j GAAAAGCACCATGGGAAATCCCTTGGCTTCTG(+CAGTGT)GTACCAGCATGGTAATTGCAAGGGAAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240605180928_mlfx TCTGAATTTCTTCCCTTGCAATTACCATGCTG(+CAGTGT)GTACCAGAAGCCAAGGGATTTCCCATGGTGC NGG 10 8 17 8 24 0 100 5 10
    240605180926_av5z AGAACTTGGGTCTGCTTTCTACAGTCATTATG(+CAGTGT)GTACCAAAACCATATGATTCTTTGTACAGTT NGG 10 8 17 8 24 0 100 5 10
    240605180925_gmtv GGATAAACTGTACAAAGAATCATATGGTTTTG(+CAGTGT)GTACCATAATGACTGTAGAAAGCAGACCCAA NGG 10 8 17 8 24 0 100 5 10
    240605180924_wgtp AGCTTGTTTCTGAGAAAACCAAGTTCTAAGTG(+CAGTGT)GTACCCAAAGGTGTTGCCAGTCCTTCCTATG NGG 10 8 17 8 24 0 100 5 10
    240605180922_6kt6 GGATGCATAGGAAGGACTGGCAACACCTTTGG(+CAGTGT)GTACCACTTAGAACTTGGTTTTCTCAGAAAC NGG 10 8 17 8 24 0 100 5 10
    240605180920_khya CTCCATGGTTAAGAACACTAGTTTTTGCTGAG(+CAGTGT)GATCCCAGTTCAATTCCCAGTACCCACATGG NGG 10 8 17 8 24 0 100 5 10