Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181029_le4u CCACTTGGACCCACACAGTTCCCCACACTCGG(+CAGTGT)GATCCCACGCCCACGCGGACTGGGACTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605181027_ekah AACACGGGCAGTCCCAGTCCGCGTGGGCGTGG(+CAGTGT)GATCCCGAGTGTGGGGAACTGTGTGGGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605181026_0af5 CCACTTGGACCCACACAGTTCCCCACACTCGG(+CAGTGT)GATCCCACGCCCACGCGGACTGGGACTGCCC NGG 10 8 17 8 24 0 100 5 10
    240605181024_706s ACCAGCTCCTCTACTTAAGGGGTACAAACAGG(+CAGTGT)GTACCCTGTTTCCCACAGGGTGACCTTACGC NGG 10 8 17 8 24 0 100 5 10
    240605181022_6tnh GGCAGGCGTAAGGTCACCCTGTGGGAAACAGG(+CAGTGT)GTACCCTGTTTGTACCCCTTAAGTAGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605181021_xo57 TTACGCCTGCCCAGCCTGAGTGGCTCTGATGG(+CAGTGT)GATCCCTTATCGAAGTGTCTCTGAGTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240605181019_ivgs GATTCAAGCCACTCAGAGACACTTCGATAAGG(+CAGTGT)GATCCCATCAGAGCCACTCAGGCTGGGCAGG NGG 10 8 17 8 24 0 100 5 10
    240605181018_5l1e AAGGGGAGGTGGATGTCTAAGTCCTCATGAAG(+CAGTGT)GATCCAGACTACTGCCTCCACCTCAAAGTCT NGG 10 8 17 8 24 0 100 5 10
    240605181017_v82t ATGAGAGACTTTGAGGTGGAGGCAGTAGTCTG(+CAGTGT)GATCCTTCATGAGGACTTAGACATCCACCTC NGG 10 8 17 8 24 0 100 5 10
    240605181016_bjzy TAAGTTAAATCCTCTGGAAGCAGATCCAAATG(+CAGTGT)GATCCACCACCTGAAACGAGGTCCCCTCTAT NGG 10 8 17 8 24 0 100 5 10