Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180947_wrj7 TTTTAATGAGGCAGTCTCAGCATTAATAAGAG(+CAGTGT)GTACCACAATTTCTGCTGGGTCTATTTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605180946_z0l3 TACTCTATTACCTGCAGGAAGAAACCCTAAAG(+CAGTGT)GTACCCAAAGAAATAAAATTTGGCAAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605180944_igtd AAACATCCACTTGCCAAATTTTATTTCTTTGG(+CAGTGT)GTACCTTTAGGGTTTCTTCCTGCAGGTAATA NGG 10 8 17 8 24 0 100 5 10
    240605180943_4ial GGTCTTGAACTCAGTATGTAGTCTACATTTTG(+CAGTGT)GATCCTCCTGCCCCCACCTCCTAAGTGTTGA NGG 10 8 17 8 24 0 100 5 10
    240605180941_igcz TAATGTCAACACTTAGGAGGTGGGGGCAGGAG(+CAGTGT)GATCCAAAATGTAGACTACATACTGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240605180940_to20 CATGAATGTTTTGGACATCAAGCATCCTAATG(+CAGTGT)GTACCTCCTTTGTACAATGCCAAAATGAGCC NGG 10 8 17 8 24 0 100 5 10
    240605180938_or3o ACCGAGGCTCATTTTGGCATTGTACAAAGGAG(+CAGTGT)GTACCATTAGGATGCTTGATGTCCAAAACAT NGG 10 8 17 8 24 0 100 5 10
    240605180937_ypq7 CTGGAGTTGGTCATTTTTGTACACCCACAGTG(+CAGTGT)GTACCTTTTACAGTCAGGTTTGGATACTTTG NGG 10 8 17 8 24 0 100 5 10
    240605180936_4q27 AGCTGCAAAGTATCCAAACCTGACTGTAAAAG(+CAGTGT)GTACCACTGTGGGTGTACAAAAATGACCAAC NGG 10 8 17 8 24 0 100 5 10
    240605180934_7un6 CCCCCAGTTCTCTGGCTGAGGCAACATTCCTG(+CAGTGT)GTACCAGTGCCTATCACTTTGTTTCAGGACT NGG 10 8 17 8 24 0 100 5 10