Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181000_5hl7 AGAACAAAACTACTGGGCCACAGGACCCTGTG(+CAGTGT)GTACCTCACAGTATGGTTCTGGAACTCCGCA NGG 10 8 17 8 24 0 100 5 10
    240605180959_d3xt GGGAATCAATTATTTTCTCCTTTAGAATTTAG(+CAGTGT)GATCCAAGTAAGATCATACAGGGGTGTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605180958_u9k0 AGTTGGGACACACCCCTGTATGATCTTACTTG(+CAGTGT)GATCCTAAATTCTAAAGGAGAAAATAATTGA NGG 10 8 17 8 24 0 100 5 10
    240605180956_j8e7 ATTTATACTTTTTAAATAAATGGTACTTGCTG(+CAGTGT)GATCCACACTAACATCTTTGCTAACAATCCC NGG 10 8 17 8 24 0 100 5 10
    240605180955_ox0o ACAATGGGATTGTTAGCAAAGATGTTAGTGTG(+CAGTGT)GATCCAGCAAGTACCATTTATTTAAAAAGTA NGG 10 8 17 8 24 0 100 5 10
    240605180954_2xjp TGAAATCAGCTCAGGTCTGAAATCGGTCTGTG(+CAGTGT)GTACCTTGGGACCCTCAGGTCAGCAGGCTGC NGG 10 8 17 8 24 0 100 5 10
    240605180952_da8t CAGCAGCAGCCTGCTGACCTGAGGGTCCCAAG(+CAGTGT)GTACCACAGACCGATTTCAGACCTGAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605180950_5z02 CCAGTATTTAACCACTCAATGCCAACTAAAAG(+CAGTGT)GTACCAGTGAAAAGTTTTGCCACAAAGAATG NGG 10 8 17 8 24 0 100 5 10
    240605180949_vxyt TTCAACATTCTTTGTGGCAAAACTTTTCACTG(+CAGTGT)GTACCTTTTAGTTGGCATTGAGTGGTTAAAT NGG 10 8 17 8 24 0 100 5 10
    240605180948_f0s7 AGTGAGCAGAAATAGACCCAGCAGAAATTGTG(+CAGTGT)GTACCTCTTATTAATGCTGAGACTGCCTCAT NGG 10 8 17 8 24 0 100 5 10