Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181014_yyy6 TATAGATAGAGGGGACCTCGTTTCAGGTGGTG(+CAGTGT)GATCCATTTGGATCTGCTTCCAGAGGATTTA NGG 10 8 17 8 24 0 100 5 10
    240605181013_dby8 CTAATATACCTCTGTTCCCAGGAGCTACTGAG(+CAGTGT)GTACCCAGTGTGGTGGGGAAAAGACGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240605181011_ojo9 CTTAATTCACCGTCTTTTCCCCACCACACTGG(+CAGTGT)GTACCTCAGTAGCTCCTGGGAACAGAGGTAT NGG 10 8 17 8 24 0 100 5 10
    240605181010_fj36 TTAAACATGAAGCCTTCCCTGTGCTGGGCATG(+CAGTGT)GATCCAGGCCAATGTTGGTGTCCTACCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605181008_wrqu TGGGCCCCAGGTAGGACACCAACATTGGCCTG(+CAGTGT)GATCCATGCCCAGCACAGGGAAGGCTTCATG NGG 10 8 17 8 24 0 100 5 10
    240605181007_xbjw CTATATCAAGCAGAACACAGGTCATCAGCTTG(+CAGTGT)GATCCATGATTTGACTTCAAGTCATTCTTTG NGG 10 8 17 8 24 0 100 5 10
    240605181006_o4rs CAGTGCAAAGAATGACTTGAAGTCAAATCATG(+CAGTGT)GATCCAAGCTGATGACCTGTGTTCTGCTTGA NGG 10 8 17 8 24 0 100 5 10
    240605181004_1kpx GCTGGGGGTACTTGCCATGAAAATTCCCTTGG(+CAGTGT)GATCCTGTGACCCTCTGGGGACAGAAGCACA NGG 10 8 17 8 24 0 100 5 10
    240605181003_eag1 ATGTGTGTGCTTCTGTCCCCAGAGGGTCACAG(+CAGTGT)GATCCCAAGGGAATTTTCATGGCAAGTACCC NGG 10 8 17 8 24 0 100 5 10
    240605181002_ix2v AATCTTGCGGAGTTCCAGAACCATACTGTGAG(+CAGTGT)GTACCACAGGGTCCTGTGGCCCAGTAGTTTT NGG 10 8 17 8 24 0 100 5 10