Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181100_70hf TAGAGTCTGTTCTCTCCAACAAACCAAAGATG(+CAGTGT)GATCCACCCAGTGCGGCTTGGTGAGTGAGCT NGG 10 8 17 8 24 0 100 5 10
    240605181058_xe2p TTGTTTCCTCCCAAATACTCCCTGAACGGGAG(+CAGTGT)GATCCCCTTCTGCTCCATCAGGACTCCACTG NGG 10 8 17 8 24 0 100 5 10
    240605181057_uwic GGCTTCAGTGGAGTCCTGATGGAGCAGAAGGG(+CAGTGT)GATCCTCCCGTTCAGGGAGTATTTGGGAGGA NGG 10 8 17 8 24 0 100 5 10
    240605181056_4dvu CCCACCACGCTCCCTAGCTGGGAGAGAAACAG(+CAGTGT)GATCCACAAGAGGAGTGGGAAAGCCGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605181054_rv59 GTGTCAAAAACGGCTTTCCCACTCCTCTTGTG(+CAGTGT)GATCCTGTTTCTCTCCCAGCTAGGGAGCGTG NGG 10 8 17 8 24 0 100 5 10
    240605181052_rey1 TAGGCTATATACCATGTTTGCAGTCAGCCTGG(+CAGTGT)GATCCATGAAACCCTGTCTCAAGAAAAGCAA NGG 10 8 17 8 24 0 100 5 10
    240605181051_oy3a TATTTTTGCTTTTCTTGAGACAGGGTTTCATG(+CAGTGT)GATCCCAGGCTGACTGCAAACATGGTATATA NGG 10 8 17 8 24 0 100 5 10
    240605181050_xlyq CTAGGGGCTGTGGAGGCTGGAGGAGAGGATCG(+CAGTGT)GATCCCTTAAATACCCATGTGGATGCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605181048_bt5e TCAGTTCCCAGCATCCACATGGGTATTTAAGG(+CAGTGT)GATCCGATCCTCTCCTCCAGCCTCCACAGCC NGG 10 8 17 8 24 0 100 5 10
    240605181047_xxuw AAGTCCTTGCTCTTAGCCAGACTAGAGATTAG(+CAGTGT)GATCCCACATGTTTATGCTGTTTGTTTTTGG NGG 10 8 17 8 24 0 100 5 10