Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181201_baxn GCGTTGGTTCTGGAAGTGTGCAGAAAGGTCAG(+CAGTGT)GATCCAGTCTACTCTTCTCCCAGCCACGCAA NGG 10 8 17 8 24 0 100 5 10
    240605181200_9dkl GTTCTGCCAAGACCGGCAGGGTAAACAAACAG(+CAGTGT)GTACCACTCTCTCCCGGGAACCCTTTCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605181158_qkaj ACAACAGAAGAAAGGGTTCCCGGGAGAGAGTG(+CAGTGT)GTACCTGTTTGTTTACCCTGCCGGTCTTGGC NGG 10 8 17 8 24 0 100 5 10
    240605181157_asuh CAGGAAAGGTTCCGTTTATAAACAAGAAACTG(+CAGTGT)GTACCAGAAAATTGCAGAGAAGCACCAACGT NGG 10 8 17 8 24 0 100 5 10
    240605181156_r4x9 GGAGCACGTTGGTGCTTCTCTGCAATTTTCTG(+CAGTGT)GTACCAGTTTCTTGTTTATAAACGGAACCTT NGG 10 8 17 8 24 0 100 5 10
    240605181154_5ay2 AAATAGGGAAAGAAGTCAGTGGCTGGTAGAGG(+CAGTGT)GTACCACTCCCCAGATGCTGTGTAGTTGAGG NGG 10 8 17 8 24 0 100 5 10
    240605181153_1zhm CAGGGCCTCAACTACACAGCATCTGGGGAGTG(+CAGTGT)GTACCCTCTACCAGCCACTGACTTCTTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605181151_wo3e CTAAGCAGCCAATGTCGCTTCTGGGGCTCCTG(+CAGTGT)GATCCTCTAGGCGGAGTCCTTTATGGTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605181150_y1c9 GGGGGGGACACCATAAAGGACTCCGCCTAGAG(+CAGTGT)GATCCAGGAGCCCCAGAAGCGACATTGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605181148_tzpy CTTTCCTTCCACACTGTCTTGTATCATGGCTG(+CAGTGT)GATCCAGTGTATTTCCAGCTAATTGTTAATG NGG 10 8 17 8 24 0 100 5 10