Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181147_enrg CATCACATTAACAATTAGCTGGAAATACACTG(+CAGTGT)GATCCAGCCATGATACAAGACAGTGTGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605181145_7lqn TATAATTTCTGTTGACTTCAAGCTTTAATTTG(+CAGTGT)GATCCACACCTACAGCTGACCCAAATGTCTC NGG 10 8 17 8 24 0 100 5 10
    240605181144_5ltp CAGAGGAGACATTTGGGTCAGCTGTAGGTGTG(+CAGTGT)GATCCAAATTAAAGCTTGAAGTCAACAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605181142_oqvc TTGGGACATTGGTTACATGGGTTATTGTATAG(+CAGTGT)GATCCTAAAATACATGTGGGGAAGAGTAATT NGG 10 8 17 8 24 0 100 5 10
    240605181141_xlmt TTTGAAATTACTCTTCCCCACATGTATTTTAG(+CAGTGT)GATCCTATACAATAACCCATGTAACCAATGT NGG 10 8 17 8 24 0 100 5 10
    240605181139_5pw2 TAGTGTGCCCCAAATGCAGCTTCACTTTCCAG(+CAGTGT)GTACCCTGATGCAGAATAAAGTTTTCATCAT NGG 10 8 17 8 24 0 100 5 10
    240605181138_p4ra GGTTTATGATGAAAACTTTATTCTGCATCAGG(+CAGTGT)GTACCTGGAAAGTGAAGCTGCATTTGGGGCA NGG 10 8 17 8 24 0 100 5 10
    240605181136_pbx3 CCTGCATTACCTGCCAGCGAGTCAAGGTAGGG(+CAGTGT)GATCCAGCAAACGGTTTTGGGAATTCAGACT NGG 10 8 17 8 24 0 100 5 10
    240605181135_1kcc TCCACAGTCTGAATTCCCAAAACCGTTTGCTG(+CAGTGT)GATCCCCTACCTTGACTCGCTGGCAGGTAAT NGG 10 8 17 8 24 0 100 5 10
    240605181133_i4ey TGAAGCACTCTGCATCCTCAGGGGAACTTGGG(+CAGTGT)GTACCCAATGGAAACATTTGTAAAAGTTCAT NGG 10 8 17 8 24 0 100 5 10