Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181216_fe0p TCGTGACTTATTTCTGCCCAAAGTGTGCTCAG(+CAGTGT)GATCCCCTGCCCTCAGGCTTAATAGACATAG NGG 10 8 17 8 24 0 100 5 10
    240605181215_ik6w TTACTTTGCGTGGCTGGGAGAAGAGTAGACTG(+CAGTGT)GATCCTGACCTTTCTGCACACTTCCAGAACC NGG 10 8 17 8 24 0 100 5 10
    240605181213_xlh9 GCGTTGGTTCTGGAAGTGTGCAGAAAGGTCAG(+CAGTGT)GATCCAGTCTACTCTTCTCCCAGCCACGCAA NGG 10 8 17 8 24 0 100 5 10
    240605181212_a5n1 GGTCTGAAAAAGTGAGGAGACACTGGAGCATG(+CAGTGT)GATCCCCAAGGTCTGAATAAGGGTTAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605181210_wpp1 ATGGACCAGCTAACCCTTATTCAGACCTTGGG(+CAGTGT)GATCCATGCTCCAGTGTCTCCTCACTTTTTC NGG 10 8 17 8 24 0 100 5 10
    240605181209_cdnk GGAGATTCATCAGAACTCAGCACACGCTGGTG(+CAGTGT)GATCCACAGCACTGTCATGTCTGTTATGAAT NGG 10 8 17 8 24 0 100 5 10
    240605181207_ynrx GTGTCATTCATAACAGACATGACAGTGCTGTG(+CAGTGT)GATCCACCAGCGTGTGCTGAGTTCTGATGAA NGG 10 8 17 8 24 0 100 5 10
    240605181206_zwj0 CCTAACCCATGCGCTGCTGGTGGGTACTGAAG(+CAGTGT)GTACCCACTTGGACTTGGAGGGGTAGGCTCC NGG 10 8 17 8 24 0 100 5 10
    240605181204_hrbx GTCATGGAGCCTACCCCTCCAAGTCCAAGTGG(+CAGTGT)GTACCTTCAGTACCCACCAGCAGCGCATGGG NGG 10 8 17 8 24 0 100 5 10
    240605181202_kvl9 TTACTTTGCGTGGCTGGGAGAAGAGTAGACTG(+CAGTGT)GATCCTGACCTTTCTGCACACTTCCAGAACC NGG 10 8 17 8 24 0 100 5 10