Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181325_xwnv TCTGTTCTGGGACTCTATACTTCCCTAACCTG(+CAGTGT)GATCCCTGCTCTGCAATGAGGTGGCCGTGGT NGG 10 8 17 8 24 0 100 5 10
    240605181323_7iwu GGCACAGTGGTAGAGCCACTCTGGATTGATGG(+CAGTGT)GATCCAATGGAATGACCTAGAGCTAAATGCA NGG 10 8 17 8 24 0 100 5 10
    240605181322_wcb3 GGGGTTGCATTTAGCTCTAGGTCATTCCATTG(+CAGTGT)GATCCCATCAATCCAGAGTGGCTCTACCACT NGG 10 8 17 8 24 0 100 5 10
    240605181319_cwvs TTTGCTCACCTGGGCAAGAAGTCATTTCCTGG(+CAGTGT)GTACCTTTCTAGCCCTGCCCATCCCAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605181318_mzmb AGAAGGAGGCTGGGATGGGCAGGGCTAGAAAG(+CAGTGT)GTACCCAGGAAATGACTTCTTGCCCAGGTGA NGG 10 8 17 8 24 0 100 5 10
    240605181317_xh99 TTCTCCCCACAGCCACAGGGAAGTTGACACTG(+CAGTGT)GATCCCTCTGCTTGTCCCCATCTTTTTTGCT NGG 10 8 17 8 24 0 100 5 10
    240605181315_8l5t AGGTGAGCAAAAAAGATGGGGACAAGCAGAGG(+CAGTGT)GATCCAGTGTCAACTTCCCTGTGGCTGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605181313_v9z7 AGCCAACAGACTTGAAAAGGCCCCTTGTCTAG(+CAGTGT)GTACCCCACGAAAATAAACTTTGAAAACGTA NGG 10 8 17 8 24 0 100 5 10
    240605181311_vgeh CTGGATACGTTTTCAAAGTTTATTTTCGTGGG(+CAGTGT)GTACCTAGACAAGGGGCCTTTTCAAGTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605181310_8rxd GAGAACAGAAGCGCCTGGGGAAGAAGGGTGAG(+CAGTGT)GATCCCCCAAATCAGAGTATGCCTGGGAGTG NGG 10 8 17 8 24 0 100 5 10