Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181425_kdp3 ATAGCCCACGTACCCTCGCAGTGTCCAGTAGG(+CAGTGT)GATCCCAGAGGCACATAACCAAGAAAGGATT NGG 10 8 17 8 24 0 100 5 10
    240605181424_vzyu TAGTAAAGCTGTCCTCCCTGCCTCTCTCACTG(+CAGTGT)GATCCGGATTGGCAACATTCCTGACTGACCC NGG 10 8 17 8 24 0 100 5 10
    240605181422_3rmk TCTGAGGGTCAGTCAGGAATGTTGCCAATCCG(+CAGTGT)GATCCAGTGAGAGAGGCAGGGAGGACAGCTT NGG 10 8 17 8 24 0 100 5 10
    240605181421_b719 CACAGCAGAACATGAATGTTCTGTATGTTTTG(+CAGTGT)GTACCCAAATCTCTAGTACACAGTACACATC NGG 10 8 17 8 24 0 100 5 10
    240605181419_0n5d GGGCAGATGTGTACTGTGTACTAGAGATTTGG(+CAGTGT)GTACCAAAACATACAGAACATTCATGTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605181417_2pjl ATTGCCATGTAGGTGCTGGGAATTGAATTCAG(+CAGTGT)GTACCCTAGAAGAACAGCCAGTGTTCTTAGC NGG 10 8 17 8 24 0 100 5 10
    240605181416_2g9c CGGGGGCTAAGAACACTGGCTGTTCTTCTAGG(+CAGTGT)GTACCTGAATTCAATTCCCAGCACCTACATG NGG 10 8 17 8 24 0 100 5 10
    240605181414_qkln TCTTCTCAATATCCGTCTACACATGGCATTGG(+CAGTGT)GTACCATCCCTCTCAGTGTCTATTGTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605181412_uxis CTCTGAGAGAACAATAGACACTGAGAGGGATG(+CAGTGT)GTACCCAATGCCATGTGTAGACGGATATTGA NGG 10 8 17 8 24 0 100 5 10
    240605181411_5k5u CTGGTTTACCTATGGAGGGAAGTGCTAGAATG(+CAGTGT)GATCCTGGACTAAACTCCCCCTGATCACCAC NGG 10 8 17 8 24 0 100 5 10