Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181354_pswg TTAATGCTCCAGCTTCTTGTTTCAATGTTTGG(+CAGTGT)GATCCACACATGATTTGAGCTACTCCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605181353_4dp1 AGCACCCTTGTGTCTCTGGCTTCCTGTCACTG(+CAGTGT)GATCCAGTACCCCTCCATGCTTGGGTCCTTG NGG 10 8 17 8 24 0 100 5 10
    240605181352_89xn TAAAACAAGGACCCAAGCATGGAGGGGTACTG(+CAGTGT)GATCCAGTGACAGGAAGCCAGAGACACAAGG NGG 10 8 17 8 24 0 100 5 10
    240605181350_2nck CTTAATATTTTCAAACTAATAGTTTATTATAG(+CAGTGT)GTACCATAAATCTTTAAATAAACACTATTTT NGG 10 8 17 8 24 0 100 5 10
    240605181349_d2vj AAAATAAAATAGTGTTTATTTAAAGATTTATG(+CAGTGT)GTACCTATAATAAACTATTAGTTTGAAAATA NGG 10 8 17 8 24 0 100 5 10
    240605181348_76la TGGAATGTCTTGGAGGCCAGAGGAGGGTGTTG(+CAGTGT)GATCCCTTAGGACTGGAGTTAAAGACGAGAG NGG 10 8 17 8 24 0 100 5 10
    240605181347_6p7w CTCACCTCTCGTCTTTAACTCCAGTCCTAAGG(+CAGTGT)GATCCAACACCCTCCTCTGGCCTCCAAGACA NGG 10 8 17 8 24 0 100 5 10
    240605181345_6rsp ATCTAGGCTCTGGTCCTCATCTGGGGTCAAAG(+CAGTGT)GATCCCTTAGGAGGGGTCAGTTTTGCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240605181344_b9k9 ACAATCAGAAGCAAAACTGACCCCTCCTAAGG(+CAGTGT)GATCCTTTGACCCCAGATGAGGACCAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240605181342_oevq TTTAAAGAACCACAGTGTGCAAATGTTTCAGG(+CAGTGT)GATCCTAAGCAATGCCCCAACACATGAACAG NGG 10 8 17 8 24 0 100 5 10