Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181341_ab8i CCAGCCTGTTCATGTGTTGGGGCATTGCTTAG(+CAGTGT)GATCCCTGAAACATTTGCACACTGTGGTTCT NGG 10 8 17 8 24 0 100 5 10
    240605181340_ia4k CCTGTAAGTACTCCCTGAGAATGCCCATTCAG(+CAGTGT)GATCCCCTGAAAGGCAAATGACAGCTATACA NGG 10 8 17 8 24 0 100 5 10
    240605181338_govy GGTCATGTATAGCTGTCATTTGCCTTTCAGGG(+CAGTGT)GATCCTGAATGGGCATTCTCAGGGAGTACTT NGG 10 8 17 8 24 0 100 5 10
    240605181337_a69d GATTAGTTTAACTCACAGGTACTGTCTACTTG(+CAGTGT)GTACCATGCAGAAACTTTCATGCAGAATGCA NGG 10 8 17 8 24 0 100 5 10
    240605181336_ij0q CTCTTTGCATTCTGCATGAAAGTTTCTGCATG(+CAGTGT)GTACCAAGTAGACAGTACCTGTGAGTTAAAC NGG 10 8 17 8 24 0 100 5 10
    240605181333_kngp TTTAATCTTTTCCCTCTGTCAGAGTACCCAGG(+CAGTGT)GATCCATGAAGTGCCTGTCGCTCGAAGACTC NGG 10 8 17 8 24 0 100 5 10
    240605181332_7hgz GCCTGGAGTCTTCGAGCGACAGGCACTTCATG(+CAGTGT)GATCCCTGGGTACTCTGACAGAGGGAAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605181330_knwt CCCACTTCTCAGGAATGACCTTCCACAGCTGG(+CAGTGT)GTACCCCTTAAATCTGCTATGTTCCTGCCTC NGG 10 8 17 8 24 0 100 5 10
    240605181328_91su ACCTGGAGGCAGGAACATAGCAGATTTAAGGG(+CAGTGT)GTACCCAGCTGTGGAAGGTCATTCCTGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605181327_8bou GTGTAACCACGGCCACCTCATTGCAGAGCAGG(+CAGTGT)GATCCAGGTTAGGGAAGTATAGAGTCCCAGA NGG 10 8 17 8 24 0 100 5 10