Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181454_eg8q CTGAAACCACGTGAGATGAGCCAACAGCCCCG(+CAGTGT)GATCCACACATGTCTGTCTCCTCTGCCTTAA NGG 10 8 17 8 24 0 100 5 10
    240605181452_12si ATGTGTGTGTGGGTGGGTTCGTGCCTGTGTTG(+CAGTGT)GTACCTGAAGACTGAACCCAGGACCTTGTGC NGG 10 8 17 8 24 0 100 5 10
    240605181451_vjqh GGCACGCACAAGGTCCTGGGTTCAGTCTTCAG(+CAGTGT)GTACCAACACAGGCACGAACCCACCCACACA NGG 10 8 17 8 24 0 100 5 10
    240605181449_fz5t CCTTGACCAAAGATGCTGAAATACAGTGTTGG(+CAGTGT)GATCCTTGTACTCTGGAACCATGCCATCACT NGG 10 8 17 8 24 0 100 5 10
    240605181448_pf11 ACATCAGTGATGGCATGGTTCCAGAGTACAAG(+CAGTGT)GATCCCAACACTGTATTTCAGCATCTTTGGT NGG 10 8 17 8 24 0 100 5 10
    240605181447_55gv AATTTTCCACTCCCGCGTTAGTCTAGTGTATG(+CAGTGT)GATCCATGTGCCCTCTATTAAGCATTACCCT NGG 10 8 17 8 24 0 100 5 10
    240605181445_eoty ACAATAGGGTAATGCTTAATAGAGGGCACATG(+CAGTGT)GATCCATACACTAGACTAACGCGGGAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605181444_p23x GGTGGGGGTAACACCTGAGCTAAGAGTGGCAG(+CAGTGT)GTACCTAGAATCCCAGGTGACTGCTTGTGTA NGG 10 8 17 8 24 0 100 5 10
    240605181443_k6w2 ACAGATACACAAGCAGTCACCTGGGATTCTAG(+CAGTGT)GTACCTGCCACTCTTAGCTCAGGTGTTACCC NGG 10 8 17 8 24 0 100 5 10
    240605181441_pkz7 CAGTGATGCCTAGAAAGATGCAGGGATCTTGG(+CAGTGT)GATCCTGTGGTCTTTACTCCTAATCAGAGCT NGG 10 8 17 8 24 0 100 5 10