Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181540_nu6p CACTTATGTAGCCCAGACTGCTCTTGAATTCG(+CAGTGT)GATCCACCACCTGTCTCTGCCTCCTGAGTGC NGG 10 8 17 8 24 0 100 5 10
    240605181539_gob3 CACTGTAGCTGACACACCAGAAGAGGGTACTG(+CAGTGT)GATCCAATTACAGATGGTCATGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605181538_907r ACCACATGGTGGCTCATGACCATCTGTAATTG(+CAGTGT)GATCCAGTACCCTCTTCTGGTGTGTCAGCTA NGG 10 8 17 8 24 0 100 5 10
    240605181536_xeio GGCTGCCCCCAGTCTCCGTCCTGCGGCTCTGG(+CAGTGT)GTACCATCCTGTTCCTTTGGGCTCATTCAGC NGG 10 8 17 8 24 0 100 5 10
    240605181534_nirw AGGAGGCTGAATGAGCCCAAAGGAACAGGATG(+CAGTGT)GTACCCAGAGCCGCAGGACGGAGACTGGGGG NGG 10 8 17 8 24 0 100 5 10
    240605181533_7chb GCTGTTCGAACCCAATCACAGCTGGCAGTGCG(+CAGTGT)GATCCGGCGCGGATCAGAGACACTGACCTTA NGG 10 8 17 8 24 0 100 5 10
    240605181531_zd8w ACATATAAGGTCAGTGTCTCTGATCCGCGCCG(+CAGTGT)GATCCGCACTGCCAGCTGTGATTGGGTTCGA NGG 10 8 17 8 24 0 100 5 10
    240605181530_9utf AGTGTGGAGTATGGTTGGCAAAGACAGGATGG(+CAGTGT)GATCCAGATTGAATACAGCATGGAATGTTTT NGG 10 8 17 8 24 0 100 5 10
    240605181528_431j TAAGGAAAACATTCCATGCTGTATTCAATCTG(+CAGTGT)GATCCCATCCTGTCTTTGCCAACCATACTCC NGG 10 8 17 8 24 0 100 5 10
    240605181527_rhur ATAGTGATTAAATGAGCATTCCTCACTTTTCG(+CAGTGT)GTACCTAGCATAAAGTCTGCATGTCAGTTTA NGG 10 8 17 8 24 0 100 5 10