Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181611_doo8 TGGGGAAGCCACCTTGTCCAGAAGTCTGTGGG(+CAGTGT)GATCCCACACAGTGGAATTTACTGCAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240605181610_l5vf TGCCCCAGGCTCCAGAGCAAGTAGGACGTCAG(+CAGTGT)GTACCTGCTCTCTTGCCTGGCTCTAGATTTT NGG 10 8 17 8 24 0 100 5 10
    240605181608_u2sj AAATTAAAATCTAGAGCCAGGCAAGAGAGCAG(+CAGTGT)GTACCTGACGTCCTACTTGCTCTGGAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605181607_o79d GCCAAGTGGGACGCATTGACTGGTCACACGTG(+CAGTGT)GTACCTCTGGCCTTTCTTTTATTTATTTATT NGG 10 8 17 8 24 0 100 5 10
    240605181605_in3e AAATAAATAAATAAATAAAAGAAAGGCCAGAG(+CAGTGT)GTACCACGTGTGACCAGTCAATGCGTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240605181604_9qwp CCCTGGGCCAAACATATTCAAACCACCATAAG(+CAGTGT)GTACCCAGAGAACAACCCCAGAACTGCACTA NGG 10 8 17 8 24 0 100 5 10
    240605181602_rr0h TCTCATAGTGCAGTTCTGGGGTTGTTCTCTGG(+CAGTGT)GTACCTTATGGTGGTTTGAATATGTTTGGCC NGG 10 8 17 8 24 0 100 5 10
    240605181601_tu27 CTTAGTCCTGAGACTGCCAGACAATCAAGCAG(+CAGTGT)GTACCCCACCAGGAGGCCAGCTCTGGACCTC NGG 10 8 17 8 24 0 100 5 10
    240605181559_lr56 TGGCAGAGGTCCAGAGCTGGCCTCCTGGTGGG(+CAGTGT)GTACCTGCTTGATTGTCTGGCAGTCTCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605181557_ujh6 CTTAGGGTTTTCTTTGCTCCTTTGTAAGGTGG(+CAGTGT)GATCCTCTTGCCAGTTTTCTCCAGCTGTGCC NGG 10 8 17 8 24 0 100 5 10