Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181628_nnfc GCCACTGGAGGGTTGGGCCTGCCCAACTCAGG(+CAGTGT)GATCCCAGCTGGAGACATTCTAGCCTTGGGT NGG 10 8 17 8 24 0 100 5 10
    240605181627_3m8q AATCACATTCTCTGTTCAGCCGTTAATGCTGG(+CAGTGT)GATCCATATTTAGTTTTATAAGCTTTTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605181625_aoa2 AAAACAGGGAAAAGCTTATAAAACTAAATATG(+CAGTGT)GATCCCAGCATTAACGGCTGAACAGAGAATG NGG 10 8 17 8 24 0 100 5 10
    240605181623_04j1 TAGTCACTTCCCTCACCAGTGCAGGTGGGTGG(+CAGTGT)GTACCAACCATGCCTCCTCCCCCCCTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240605181622_4ep2 CCCCCAAAAAAGGGGGGGAGGAGGCATGGTTG(+CAGTGT)GTACCCACCCACCTGCACTGGTGAGGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605181620_g6hm GGGCAAGCCGGTTCTCTCCTTCCACCAGATGG(+CAGTGT)GATCCCAGGGGATTGAATTCAGGTTATCTGG NGG 10 8 17 8 24 0 100 5 10
    240605181618_car3 AAATCCCAGATAACCTGAATTCAATCCCCTGG(+CAGTGT)GATCCCATCTGGTGGAAGGAGAGAACCGGCT NGG 10 8 17 8 24 0 100 5 10
    240605181616_cpjx CCTGTGACCCTGCATCCCATGGGTCCTGTGAG(+CAGTGT)GTACCCGAGCCCCCTTTCTAACTCCCACCTC NGG 10 8 17 8 24 0 100 5 10
    240605181615_p3s9 TTGGGGAGGTGGGAGTTAGAAAGGGGGCTCGG(+CAGTGT)GTACCTCACAGGACCCATGGGATGCAGGGTC NGG 10 8 17 8 24 0 100 5 10
    240605181613_mraz CTTTGTCCACTGCAGTAAATTCCACTGTGTGG(+CAGTGT)GATCCCCACAGACTTCTGGACAAGGTGGCTT NGG 10 8 17 8 24 0 100 5 10