Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181702_fjib GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+CAGTGT)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    240605181700_5ugn CCTTCTACCAAGTGGGTAGTCCGTCCTGATAG(+CAGTGT)GTACCTTTACCTGCTGAGCCATCTGAGTAGC NGG 10 8 17 8 24 0 100 5 10
    240605181659_r2jg GTGAGGCTACTCAGATGGCTCAGCAGGTAAAG(+CAGTGT)GTACCTATCAGGACGGACTACCCACTTGGTA NGG 10 8 17 8 24 0 100 5 10
    240605181657_ox19 CAGTGGGTTGCTGCTCTCCAGGGATGTGGCTG(+CAGTGT)GTACCCCAGGTTCTTCCAGGTCATTCTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605181655_ocu3 AGAGGCAGGAGAATGACCTGGAAGAACCTGGG(+CAGTGT)GTACCAGCCACATCCCTGGAGAGCAGCAACC NGG 10 8 17 8 24 0 100 5 10
    240605181654_piak GTGGACTGCATCCGACTGAAATAAGCAAAATG(+CAGTGT)GTACCACAGCGCCCGGGTGCACACAGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240605181653_2w5y TTCATGGCTGCTGTGTGCACCCGGGCGCTGTG(+CAGTGT)GTACCATTTTGCTTATTTCAGTCGGATGCAG NGG 10 8 17 8 24 0 100 5 10
    240605181651_5grt GTAAGACTGGGAGAAAGATTTCTTAACCAGTG(+CAGTGT)GTACCTGACTCTTGACCTGGTCATGGTCCTG NGG 10 8 17 8 24 0 100 5 10
    240605181650_uihk ATTCCCAGGACCATGACCAGGTCAAGAGTCAG(+CAGTGT)GTACCACTGGTTAAGAAATCTTTCTCCCAGT NGG 10 8 17 8 24 0 100 5 10
    240605181649_sm0f AGAATTTTCACGGCAGGTACATAAAGGAAGTG(+CAGTGT)GTACCCAGTCTCCAGTCAGGACGTGAGCACC NGG 10 8 17 8 24 0 100 5 10