Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181806_vlgs GCTAGTCAAACCGTATCTGGGAAATGTAAGAG(+CAGTGT)GTACCCTGCCAAGGACAGAGTAAGAAAACAA NGG 10 8 17 8 24 0 100 5 10
    240605181804_t4uz ACAAATCTTACTCATACACTGATTCTTTCCTG(+CAGTGT)GATCCCTGGGAAAGGAGCAGTACACTAGACA NGG 10 8 17 8 24 0 100 5 10
    240605181802_vjrg TTAAATGTCTAGTGTACTGCTCCTTTCCCAGG(+CAGTGT)GATCCAGGAAAGAATCAGTGTATGAGTAAGA NGG 10 8 17 8 24 0 100 5 10
    240605181801_kxkz TGGCATTTGACTGGTGGAGATGGATTACATTG(+CAGTGT)GTACCATAAATGATGGAACTCTGCAATGGAA NGG 10 8 17 8 24 0 100 5 10
    240605181759_mzcf GGCCTTTCCATTGCAGAGTTCCATCATTTATG(+CAGTGT)GTACCAATGTAATCCATCTCCACCAGTCAAA NGG 10 8 17 8 24 0 100 5 10
    240605181756_tpu9 AGGGTAAACAAGGGAGACTGCTGCAGCCCCTG(+CAGTGT)GATCCCAAATGCCCATGTCTGAGCCACACGA NGG 10 8 17 8 24 0 100 5 10
    240605181755_tuza GAGCTTCGTGTGGCTCAGACATGGGCATTTGG(+CAGTGT)GATCCAGGGGCTGCAGCAGTCTCCCTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240605181752_gnm0 AGGGTAAACAAGGGAGACTGCTGCAGCCCCTG(+CAGTGT)GATCCCAAATGCCCATGTCTGAGCCACACGA NGG 10 8 17 8 24 0 100 5 10
    240605181750_7ven GAGCTTCGTGTGGCTCAGACATGGGCATTTGG(+CAGTGT)GATCCAGGGGCTGCAGCAGTCTCCCTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240605181749_f3x0 CAAAGGCTCCAGTTCCTACAAGTTGGTAGAGG(+CAGTGT)GATCCAGGTGTACTTTCAGTCACTGGAAGAA NGG 10 8 17 8 24 0 100 5 10