Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181747_y68m TGTAATTCTTCCAGTGACTGAAAGTACACCTG(+CAGTGT)GATCCCTCTACCAACTTGTAGGAACTGGAGC NGG 10 8 17 8 24 0 100 5 10
    240605181746_eosn TGTCCGGGTCCCATGTGAGTGACTCTAGGAAG(+CAGTGT)GATCCTAGGATTGTGAAGTGAACAGTAACAC NGG 10 8 17 8 24 0 100 5 10
    240605181744_2c8o GACAAGTGTTACTGTTCACTTCACAATCCTAG(+CAGTGT)GATCCTTCCTAGAGTCACTCACATGGGACCC NGG 10 8 17 8 24 0 100 5 10
    240605181742_5kbh ACACCGAGGAACCCCAGTTAGGAAGATCTGGG(+CAGTGT)GTACCAGAGAATGCTTGCCCTTTCCTTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605181741_hd1l GCCAACCTGAAGGAAAGGGCAAGCATTCTCTG(+CAGTGT)GTACCCCAGATCTTCCTAACTGGGGTTCCTC NGG 10 8 17 8 24 0 100 5 10
    240605181739_fd7u CAGTGAGCATCCTGCCTGCCTATGCATTTTGG(+CAGTGT)GTACCTCCCCTGTGACACCCACCCCCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240605181737_u9da ATACAGCTCTGGGGGTGGGTGTCACAGGGGAG(+CAGTGT)GTACCCAAAATGCATAGGCAGGCAGGATGCT NGG 10 8 17 8 24 0 100 5 10
    240605181736_o04z CTTGTATACTGCAAGACAGGTCACACACCAAG(+CAGTGT)GATCCTTCAAACACAAACGCTCCTCCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605181734_8ovp ATCAAGAAGGGGAGGAGCGTTTGTGTTTGAAG(+CAGTGT)GATCCTTGGTGTGTGACCTGTCTTGCAGTAT NGG 10 8 17 8 24 0 100 5 10
    240605181733_i1jh TCAAGAGGCAAAGTCTCCACTTTCCTACACAG(+CAGTGT)GATCCATCTTTTGTCTCAGCCAACTCTCAAT NGG 10 8 17 8 24 0 100 5 10