Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181732_kb4v CCTCCATTGAGAGTTGGCTGAGACAAAAGATG(+CAGTGT)GATCCTGTGTAGGAAAGTGGAGACTTTGCCT NGG 10 8 17 8 24 0 100 5 10
    240605181730_xw4u GGGAAGAACTGTTAAGTATCGCTAGGGTCAGG(+CAGTGT)GATCCTGACACCTTGGTCAGCTACCAAACAC NGG 10 8 17 8 24 0 100 5 10
    240605181729_l0eh CAAGAGTGTTTGGTAGCTGACCAAGGTGTCAG(+CAGTGT)GATCCCTGACCCTAGCGATACTTAACAGTTC NGG 10 8 17 8 24 0 100 5 10
    240605181728_8gdj GCTCCAGTGCCTACAGCTGCAGGGTGGCATTG(+CAGTGT)GATCCTGTGACTCCCGGTCCTTCCTAAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605181726_awx8 TCCTTTCACTTAGGAAGGACCGGGAGTCACAG(+CAGTGT)GATCCAATGCCACCCTGCAGCTGTAGGCACT NGG 10 8 17 8 24 0 100 5 10
    240605181725_c69r TTTAATTAAAGAGAATTCTTCCACTTCGTCGG(+CAGTGT)GATCCGTCTTCTGCAATCAAAGTGGACTGCT NGG 10 8 17 8 24 0 100 5 10
    240605181724_d36e CCTCAAGCAGTCCACTTTGATTGCAGAAGACG(+CAGTGT)GATCCCGACGAAGTGGAAGAATTCTCTTTAA NGG 10 8 17 8 24 0 100 5 10
    240605181722_qqrg TGTGCTGTCACCACTCCCCAATGTCCTGGGAG(+CAGTGT)GTACCTCCAGTCAGAGGCAAAGAGGCCATGA NGG 10 8 17 8 24 0 100 5 10
    240605181721_ibuy CTCTGTCATGGCCTCTTTGCCTCTGACTGGAG(+CAGTGT)GTACCTCCCAGGACATTGGGGAGTGGTGACA NGG 10 8 17 8 24 0 100 5 10
    240605181719_dizq TGTGGGGTGGGCTCTGGAGGCAGGCGACAGTG(+CAGTGT)GTACCAGGCTGCCTACTGAGTGTCTATGGGG NGG 10 8 17 8 24 0 100 5 10