Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605181526_rf35 AGCAATAAACTGACATGCAGACTTTATGCTAG(+CAGTGT)GTACCGAAAAGTGAGGAATGCTCATTTAATC NGG 10 8 17 8 24 0 100 5 10
    240605181524_t0nj TTCCAAGTGAAGTCTGCTAAGCAGACCTCAGG(+CAGTGT)GTACCTGGCAGAGCCTGTGTGAACTGGGTGT NGG 10 8 17 8 24 0 100 5 10
    240605181522_w607 CATTCACACCCAGTTCACACAGGCTCTGCCAG(+CAGTGT)GTACCCTGAGGTCTGCTTAGCAGACTTCACT NGG 10 8 17 8 24 0 100 5 10
    240605181521_p8m4 GTGCAAATTTCCAGAAAATAATGGAGATACAG(+CAGTGT)GTACCATAGGACAGTGAAGCAAGAATGTCTC NGG 10 8 17 8 24 0 100 5 10
    240605181519_gf97 CTGGAGAGACATTCTTGCTTCACTGTCCTATG(+CAGTGT)GTACCTGTATCTCCATTATTTTCTGGAAATT NGG 10 8 17 8 24 0 100 5 10
    240605181518_b57w GTTTTAGTTTACAAAAGAAAAATCAATGATTG(+CAGTGT)GTACCTTTTTTTAACACTCTCAGATTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605181517_be41 CATATTCAGGAATCTGAGAGTGTTAAAAAAAG(+CAGTGT)GTACCAATCATTGATTTTTCTTTTGTAAACT NGG 10 8 17 8 24 0 100 5 10
    240605181514_6esw TGAAGACAATAAATATTCCTTTGAGACCCGTG(+CAGTGT)GATCCTTCTGCGGACCCTAATGACACAAAGT NGG 10 8 17 8 24 0 100 5 10
    240605181513_6tyy GCTCCACTTTGTGTCATTAGGGTCCGCAGAAG(+CAGTGT)GATCCACGGGTCTCAAAGGAATATTTATTGT NGG 10 8 17 8 24 0 100 5 10
    240605181512_hcnm ACCGCTCTCAAAGTAGACGGCATCGCTGCGTG(+CAGTGT)GATCCACGCCGCTCACGTAAAAGCGGCGACA NGG 10 8 17 8 24 0 100 5 10