Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605180544_97nf AAACCTTAAGGATGGGGTACACAGGGGAGCAG(+CAGTGT)GTACCCATGATGCTGGCCCGGTCAGAGAATT NGG 10 8 17 8 24 0 100 5 10
    240605180542_1gxe ATGGTGAAGGGAAGTCGGGAGGGGGGGAAGGG(+CAGTGT)GATCCGAACACAGTTGACTTTTATTTTGTAA NGG 10 8 17 8 24 0 100 5 10
    240605180541_r0gg TCTTTTTACAAAATAAAAGTCAACTGTGTTCG(+CAGTGT)GATCCCCTTCCCCCCCTCCCGACTTCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605180539_myhb ACAGACAACTGAGTTGCCCATTACACAACGGG(+CAGTGT)GATCCACTCCAACAGATGAGCCCCTGATGGG NGG 10 8 17 8 24 0 100 5 10
    240605180538_lylh TGACTCCCATCAGGGGCTCATCTGTTGGAGTG(+CAGTGT)GATCCCCGTTGTGTAATGGGCAACTCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605180536_f0ff AAAAAGCCCTCATGACACAGAGCCTTCCTCGG(+CAGTGT)GATCCTGATCTTAGCGTTCTACTCTGAATCC NGG 10 8 17 8 24 0 100 5 10
    240605180535_u1ia CTCTGGGATTCAGAGTAGAACGCTAAGATCAG(+CAGTGT)GATCCCGAGGAAGGCTCTGTGTCATGAGGGC NGG 10 8 17 8 24 0 100 5 10
    240605180533_xiz8 TGTGCTCACTCCCTGGTGAACAGAGGAGCAGG(+CAGTGT)GATCCCAGAAAATAAAACGTGGAGGTCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605180532_ozhe TGGAAAAGAGACCTCCACGTTTTATTTTCTGG(+CAGTGT)GATCCCTGCTCCTCTGTTCACCAGGGAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605180531_2ctp CAGTGAGCAGCATATTTTATATACTGGTTGAG(+CAGTGT)GATCCCTAGTGAACATTATGACATCTGAACT NGG 10 8 17 8 24 0 100 5 10